MOLECULAR CHARACTERIZATION OF PANDEMIC INFLUENZA VIRUSES A/H1N1(sw2009) ISOLATED IN RUSSIA IN 2009 — 2010


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Abstract

Aim . Assessment of genetic diversity of influenza virus A/H1N1(sw2009) variants circulated in Russia, study of virus’ pathogenicity in humans and potential resistance to antiviral drugs. Materials and methods. Sequencing of PCR-fragments of genome of influenza viruses isolated from clinical and autopsy samples of 436 patients. Four full genome sequences of influenza viruses A/H1N1(sw2009) were obtained. Phylogenetic analysis was performed. Results. High degree of homology (98.9—100%) was found among influenza A/H1N1(sw2009) viruses in HA and NA genes as well as in their aminoacid sequences (1.3 and 1.4% respectively). Differences in other proteins did not exceed 1.1%. Diversity was found in position 222 of receptor-binding locus of HA and single amino acid polymorphism — in several internal proteins. Known mutations determining resistance to Tamiflu and Arbidol were not detected. All viruses were resistant to remantadine. Molecular markers of high pathogenicity were not found. Conclusion. High homology of influenza viruses determines low level of antigenic differences although in populations of viruses there are variants with different levels of adaptation to human organism and different affinity to receptors of upper and lower respiratory tract that can determine their different transmissibility.

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МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКАЯ ХАРАКТЕРИСТИКА ВИРУСОВ ПАНДЕМИЧЕСКОГО ГРИППА A/H1N1(sw2009), ИЗОЛИРОВАННЫХ В 2009 — 2010 ГГ. В РОССИИ
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About the authors

S. B Yatsyshina

Central Research Institute of Epidemiology, Moscow, Russia

A. N Minenko

Central Research Institute of Epidemiology, Moscow, Russia

P. V Voloshina

Central Research Institute of Epidemiology, Moscow, Russia

A. V Kudryavtseva

Central Research Institute of Epidemiology, Moscow, Russia

M. N Praded

Central Research Institute of Epidemiology, Moscow, Russia

S. I Braslavskaya

Central Research Institute of Epidemiology, Moscow, Russia

G. A Shipulin

Central Research Institute of Epidemiology, Moscow, Russia

V. V Maleev

Central Research Institute of Epidemiology, Moscow, Russia

V. I Pokrovsky

Central Research Institute of Epidemiology, Moscow, Russia

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Copyright (c) 2011 Yatsyshina S.B., Minenko A.N., Voloshina P.V., Kudryavtseva A.V., Praded M.N., Braslavskaya S.I., Shipulin G.A., Maleev V.V., Pokrovsky V.I.

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