EVALUATION OF TOXIN PRODUCING ABILITIES OF NON-O1/NON-O139 SEROGROUP VIBRIO CHOLERAE ISOLATED FROM HUMANS

Aim. Determination of non-O1/non-O139 Vibri o cholerae toxin (CT) gene expression by using EIA, and biological effect of non-O1/non-O139 V.cholerae supernatant on cell cultures evaluation. M aterials and methods. 39 V.cholerae strains from various serological groups were studied. Hemolytic activity of strains was determined by using Greig test, and cholera toxin production — in GM1-EIA and in continuous cell lines by registering cytotonic, cytotoxic and proteolitic effect. Results. GM1-EIA method does not detect CT production in 29 museum strains of non-O1/non-O139 V.cholera e in vitro . CT was detected only in 1 non-O1/non-O139 V.cholerae strain supernatant with OD=0.577 that is substantially lower than in O1 V.cholerae strains (OD=2.176). In cell cultures non-O1/non-O139 V.cholerae supernatants diluted to 1:100 caused elongation only in single cells. Conclusion. Cytological model is a more sensitive technique to evaluate toxin producing abilities of non-O1/non-O139 V.cholerae strains and is appropriate for use.

Vibrio cholerae non-O1/non-O139, cholera toxin, GM1-EIA, continuous cell cul- tures