CHRONIC PERSISTENT INFECTIONS OF ANTERIOR SEGMENT OF EYE: CLINICOLABORATORY ASPECTS

Aim. Improvement of therapy of chronic ophthalmic infectious diseases during assessment of functioning of different arms of immune system. Materials and methods. Three hundred and fifty patients with chronic red-eye syndrome were tested by immunofluorescence assay on the presence of antigens of herpesviruses, adenoviruses and Chlamydia in samples from conjunctiva. Expression of 11 cytokines ' genes was measured in peripheral blood mononuclear cells by reverse transcription polymerase chain reaction. Production of IFN-α and IFN-γ, levels of serum and spontaneously produced interferon as well as level of susceptibility to the range of immunomodulating preparations were measured during study of interferon status in whole blood cells. Study of parameters of cytokine, interferon and immune statuses was performed in 70 patients. Counts of Tand B-lymphocytes, T-helpers, NK-cells as well as level of circulating immune complexes were measured during study of immune status. Results. Antigens of herpes simplex virus and adenoviruses were detected in samples from conjunctiva in 27% (95 persons) and 36% (126 persons) of patients respectively. Enhanced level of expression of several cytokines (IL-2, IL-4) in studied patients compared with healthy volunteers was observed. Expression levels of IL-12 and TNF-α mRNAs were, in opposite, in 2 — 3 times lower. Disorder of IFN-α and IFN-γ synthesis on post-transciption level was observed in 60 — 90% of patients. Decrease of absolute numbers of total T-lymphocytes and T-helpers as well as increase of absolute number of NK-cells was noted in 20%, 25%, and 27% of patients respectively. Conclusion. Assignment of individually oriented antiviral, antibacterial and immunomodulating therapy allowed to mitigate intensity of clinical symptoms in 30 — 60% of patients with chronic persistent infections of anterior segment of eye.

red-eye syndrome, ophtalmoherpes, adenovirus infection of eyes, ophtalmochlamydiosis, complex examination, cytokine profile, interferon status, immune status