Email this article STUDY OF PROTECTIVE PROPERTIES OF RECOMBINANT ATOXIC FORM OF EXOTOXIN A AND RECOMBINANT OUTER MEMBRANE PROTEIN F OF PSEUDOMONAS AERUGINOSA
- Authors: Mikhaylova N.A1, Vertiev Y.V1, Kaloshin A.A1, Isakov M.A1
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Affiliations:
- Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
- Issue: Vol 87, No 2 (2010)
- Pages: 39-44
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13433
- ID: 13433
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Abstract
Aim. To obtain recombinant atoxic form of Pseudomonas aeruginosa exotoxin A and assess its protective properties during simultaneous administration with recombinant protein F in experiment. Materials and methods. Genetic methods were employed for construction of deletion variant of P.aeruginosa exotoxin A gene, whereas Escherichia coli cells were used for transformation. Purification of proteins was performed by common method. White outbred mice were used for immunization and experimental infection was produced by intraperitoneal administration of live virulent culture of P.aeruginosa strain PA103. Results. The gene coding defect form of P.aeruginosa exotoxin A with lacked 106 C-terminal aminoacid residues was cloned. Synthesized protein was nontoxic and immunogenic. Recombinant variants of anatoxin A and outer membrane protein F of P.aeruginosa protected animals from experimental infection caused by toxigenic strain PA103 of P.aeruginosa if were administered separately or concomitantly. Nonetheless, the highest protective effect was observed after immunization with both proteins. Conclusion. Studied recombinant toxoid and OprF could be the candidates for inclusion in vaccines for prevention of pseudomonas infection.
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ИССЛЕДОВАНИЕ ПРОТЕКТИВНЫХ СВОЙСТВ РЕКОМБИНАНТНОЙ АТОКСИЧНОЙ ФОРМЫ ЭКЗОТОКСИНА АИ РЕКОМБИНАНТНОГО БЕЛКА F НАРУЖНОЙ МЕМБРАНЫ PSEUDOMONAS AERUGINOSA×
About the authors
N. A Mikhaylova
Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
Yu. V Vertiev
Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
A. A Kaloshin
Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
M. A Isakov
Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
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