Email this article ULTRASTRUCTURAL CHANGE OF LACTOBACILLI DURING SUPPRESSION OF THEIR GROWTH BY CLINICAL STRAINS OF CANDIDA ALBICANS
- Authors: Bondarenko V.M1,2, Rybalchenko O.V1,2
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Affiliations:
- Gamaleya Research Institute of Epidemiology and Microbiology, Moscow
- State Research Institute of High Pure Biopreparations, Saint-Petersburg, Russia
- Issue: Vol 86, No 4 (2009)
- Pages: 96-99
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13384
- ID: 13384
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Abstract
Aim. Detection of morphophysiological changes in cells of manufacturing strains of lactobacilli sup- pressed by antagonistically active clinical cultures of Candida albicans , isolated from women with torpid course of vaginosis and vaginitis. Materials and methods. Three clinical strains of C.albicans suppressing growth of bacterial cells from manufacturing strains of Lactobacillus plantarum 8-PA-3, L.fermentum 90-4С and L.acidophilus Д75 on modified agar-added nutrient medium were used. Using electron microscopy, ultrastructural changes in lactobacilli suppressed by C.albicans were studied. Results. In places of tight contacts of interacting microorganisms, destructive changes in cell walls of lactobacilli appeared as desquamation of small layerwise fragments of peptidoglycan layers were observed. Rearrangement of ultrafine structure of lactobacilli's cytoplasm which expressed as des- truction of protein-ribosomal complex was also noted. Local defects of cellular components led to total destruction of cytoplasmic contents and appearance of «cellular shadows». Conclusion. Surmounting by C.albicans the antagonistic action of lactobacilli with destruction of peptidoglycan layer of their cell walls could explain persistence of yeast fungi during chronic forms of vaginosis in women.
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УЛЬТРАСТРУКТУРНЫЕ ИЗМЕНЕНИЯ ЛАКТОБАЦИЛЛ ПРИ ПОДАВЛЕНИИ ИХ РОСТА КЛИНИЧЕСКИМИ ШТАММАМИ СANDIDA ALBICANS×
About the authors
V. M Bondarenko
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow; State Research Institute of High Pure Biopreparations, Saint-Petersburg, Russia
O. V Rybalchenko
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow; State Research Institute of High Pure Biopreparations, Saint-Petersburg, Russia
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