FORMATION OF BIOFILMS BY FECAL STRAINS OF ENTEROBACTERIA AND YEAST FUNGI FROM CANDIDA GENUS

Aim. To assess the ability of bacteria and Candida genus' yeast fungi from human fecal microflora to form biofilms. Materials and methods. Thirty-one strains of enterobacteria and 45 strains of yeast fungi from Candida genus isolated from feces of patients during test on intestinal dysbiosis were used. In order to study the ability of microorganisms to form biofilms, level of binding of crystal violet by the formers in sterile 96-well polystyrene plates was studied. Results. Ability to form biofilms was noted in 87.1% of enterobacteria strains. This characteristic was observed in lactose-positive and lactose-negative Escherichia coli and bacteria belonging to Klebsiella , Citrobacter , Serratia , Morganella , Providencia genuses. The highest mean value of the characteristic was noted in strains belonging to Enterobacter cloacae species (6.52±2.01). Overwhelming majority of fungal strains belonging to Candida genus formed static biofilms in wells of polystyrene plates. Mean value of the characteristic in group of Candida spp. (not albicans ) was significantly higher compared with strains from C.albicans species (2.75±0.53 vs. 1.38±0.11, p<0.05). The highest value of the mentioned characteristic (6.75±1.65) was observed in yeast fungi belonging to C.krusei species. Conclusion. Obtained data widen our knowledge about armamentarium of biological characteristics of opportunistic microorganisms (bacteria and fungi) promoting their prolonged persistence in intestinal biotope.

biofilms, gut microflora, dysbiosis, enterobacteria, yeast fungi, persistence