RECOMBINANT HEAT-SHOCK PROTEIN (rHSP70) BOOSTS ACTIVATION OF INNATE AND ADAPTIVE IMMUNITY DURING SIMULTANEOUS ADMINISTRATION WITH BACTERIAL ANTIGENS IN EXPERIMENT


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Abstract

Experimental study of adjuvant effect of recombinant mycobacterial heat-shock protein rHSP70 on immune response to antigens of opportunistic microorganisms was performed. Therapeutic polycomponent vaccine Immunovac-VP-4, containing ligands for binding with Toll-like receptors (TLRs) 2, 4 and 9, was used as a source of bacterial antigens. Obtained data showed that administration of rHSP70 mixed with bacterial antigens of opportunistic microorganisms leads to maturation of mice dendritic cells obtained from bone marrow precursors, increased expression of TLRs 2, 4, 9 on their surface and production of cytokines IL 1 β , IL-6, IL-10, TNF α , as well as to activation of innat e immunity in experiments in vivo resulting to increased resistance of mice to experimental Salmonella infection. Simultaneous administration of rHSP70 with bacterial antigens increased titers of antibodies to vaccine’s antigenic components in direct hemagglutination reaction. Thus, adjuvant effect of rHSP70 was confirmed on the used model as well as increase of stimulation of innate and adaptive immunity was shown.

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РЕКОМБИНАНТНЫЙ БЕЛОК ТЕПЛОВОГО ШОКА (rHSP70) УСИЛИВАЕТ АКТИВАЦИЮ ВРОЖДЕННОГО И АДАПТИВНОГО ИММУНИТЕТА ПРИ СОВМЕСТНОМ ВВЕДЕНИИ С БАКТЕРИАЛЬНЫМИ АНТИГЕНАМИ В ЭКСПЕРИМЕНТЕ
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About the authors

Yu. S Shevchik

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

E. A Kurbatova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

P. G Sveshnikov

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. K Akhmatova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. B Egorova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

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Copyright (c) 2009 Shevchik Y.S., Kurbatova E.A., Sveshnikov P.G., Akhmatova N.K., Egorova N.B.

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