INFLUENCE OF CULTIVATION TEMPERATURE OF YERSINIA PSEUDOTUBERCULOSIS ON THE IMMUNOBIOLOGICAL PROPERTIES OF LYPOPOLYSACCHARIDE


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Abstract

Aim. To study relationship between Yersinia pseudotuberculosis cultivation temperature and immunobiological properties of lypopolysaccharide (LPS). Materials and methods. LPS producing strain — Yersinia pseudotuberculosis 164/84 OX; experimental animal — guinea pigs, rabbits; methods — immunochemical (indirect hemagglutionation assay, RDP), physico-chemical (electrophoresis, gas-liquid chromatography), standard biochemical and statistical methods. Results. It was established that increase of Yersinia pseudotuberculosis cultivation temperature from 10°C to 37°C was associated with attenuation of LPS toxicity in guinea pigs, decrease of its immunochemical activity and contents and degree of acylation of 3OH-14:0 hydoxyacid as well as amount of electrophoretically detected O-side chains. It was assumed that the polysaccharide component plays its role in LPS toxicity. Conclusion. Relation between Yersinia pseudotuberculosis cultivation temperature and structural and functional properties of LPS preparations obtained from the culture was determined.

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ВЛИЯНИЕ ТЕМПЕРАТУРЫ КУЛЬТИВИРОВАНИЯ YERSINIA PSEUDOTUBERCULOSIS НА ИММУНОБИОЛОГИЧЕСКИЕ СВОЙСТВА ЛИПОПОЛИСАХАРИДА
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About the authors

A. A Byvalov

48 Central Research Institute of Ministry of Defense of Russian Federation, Kirov; Institute of Physiology, Syktyvkar, Russia

Yu. D Eremenko

48 Central Research Institute of Ministry of Defense of Russian Federation, Kirov; Institute of Physiology, Syktyvkar, Russia

V. I Drobkov

48 Central Research Institute of Ministry of Defense of Russian Federation, Kirov; Institute of Physiology, Syktyvkar, Russia

I. V Smirnova

48 Central Research Institute of Ministry of Defense of Russian Federation, Kirov; Institute of Physiology, Syktyvkar, Russia

L. B Gavrilova

48 Central Research Institute of Ministry of Defense of Russian Federation, Kirov; Institute of Physiology, Syktyvkar, Russia

D. V Pechenkin

48 Central Research Institute of Ministry of Defense of Russian Federation, Kirov; Institute of Physiology, Syktyvkar, Russia

A. V Kozhemyako

48 Central Research Institute of Ministry of Defense of Russian Federation, Kirov; Institute of Physiology, Syktyvkar, Russia

L. G Bobrova

48 Central Research Institute of Ministry of Defense of Russian Federation, Kirov; Institute of Physiology, Syktyvkar, Russia

References

  1. Ашмарин И.П., Воробьев А.А. Статистические методы в микробиологических исследованиях. Л., 1962.
  2. Бывалов А.А., Дармов И.В., Евстигнеев В.И., Пименов Е.В. Идентификация и выделение антигена, защищающего морских свинок от экспериментальной чумы. Пробл. особо опасных инфекций. 2005, 89: 54—58.
  3. Еременко Ю.Д., Смирнов С.Г., Чернядьев А.В. Изменение жирнокислотного состава бактерий Y.pestis штамма EV в процессе глубинного культивирования. Биопрепараты. 2002, 1: 21—23.
  4. Захарова И.Я., Косенко Л.В. Методы изучения микробных полисахаридов. Киев, Наукова думка, 1982.
  5. Книрель Ю.А., Кочетков Н.К. Строение ли пополисахарида грамотрицательных бактерий. Биохимия. 1993, 58: 166—181.
  6. Красикова И.Н., Бахолдина С.И., Соловьева Г.Ф. Синтез липополисахаридов у бактерий Yersinia pseudotuberculosis: влияние плазмиды pVM82 и температуры роста бактерий. Там же. 2000, 65 (11): 1507—1514.
  7. Методы общей бактериологии. Ф.Герхардт (ред.). М., Мир, 1984.
  8. Сомов Г.П., Пок ровск и й В.И., Бесед нова Н.М., Антоненко Ф.Ф. Псевдотуберкулез. М., Медицина, 2001.
  9. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227: 680—685.
  10. Lowry O.H., Rosenbrough N.J., Farr A.L., Randall R.J. Protein measurement with the folin phenol reagent. J.Biol. Chem. 1951, 193: 265—275.
  11. Skurnik M. Molecular genetic, biochemistry and biological role of Yersinia lipopolysaccharide. In: The genus Yersinia: entering the functional genomic era. M.Skurnik, J.A.Bengoechea, K. Granfors (ed.). Kluwer Academic Plenum Publ. 2002: р. 187—197.
  12. Tsai C.M., Frasch C.E. A sensitive silver strain for detecting lipopolysaccharide gels. Anal. Biochem. 1982, 119: 115—119.
  13. Westphal O., Jan K. Bacterial lipopolysaccharides. Methods Carbohydr. Chem. 1965, 6: 83—91.

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Copyright (c) 2009 Byvalov A.A., Eremenko Y.D., Drobkov V.I., Smirnova I.V., Gavrilova L.B., Pechenkin D.V., Kozhemyako A.V., Bobrova L.G.

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