METABOLIC PROFILE OF BIFIDOFLORA UNDER DIFFERENT MICROECO-LOGICAL CONDITIONS OF THE COLON BIOTOPE IN HUMAN

Aim. To study the spectrum and level of short-chained fatty acids (SCFA) in supernatant of bifidobacteria under different microecological conditions of the colon biotope in human. Materials and methods. Metabolites of 88 bifidobacteria strains isolated from patients when examined for dysbiosis of the colon were investigated. Definition of concentration of SCFA was performed on acidified supernatant samples by a separation method on chromatograph GC-2010 Plus, Shimadzu (Japan). Results. Monobasic acids were found in metabolites of 50 -100% study cultures of bifidobacteria where the spectrum and level of carboxylic acids in supernatants varied depending on microecological condition of the origin of the discharge. In severe damages of microsym-biocenosis in metabolites of Bifidobacterium spp., summarized concentrations of SCFA, structural index, levels of aceitic and propionic acids were decreased. Strain-specific differences in a metabolic profile of bifidoflora in a composition of individual consortiums were determined. Data obtained indicate the variation of functional (metabolic) activity of dominant strains in different microecological conditions of the human colon. Conclusion. Uniquieness of metabolome of every other strain due to their strain specifity determines their functional activity, but a metabolic profile of bifidoflora can serve as the most important criterion for the selection of effective probiotic drugs for treatment and prevention of dysbioisis in the colon.

bifidobacteria, short-chained fatty acids, metabolic profile, strain specifity, dysbiosis