FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA

D. V. Ulshina; Ульшина Д. В.; D. A. Kovalev; Ковалев Д. А.; A. M. Zhirov; Жиров А. М.; N. V. Zharinova; Жаринова Н. В.; A. A. Khudoleev; Худолеев А. А.; O. I. Kogotkova; Коготкова О. И.; V. I. Efremenko; Ефременко В. И.; N. I. Evchenko; Евченко Н. И.; A. N. Kulichenko; Куличенко А. Н.

doi:10.36233/0372-9311-2016-1-29-34

FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA

Authors: Ulshina D.V.¹, Kovalev D.A.¹, Zhirov A.M.¹, Zharinova N.V.¹, Khudoleev A.A.¹, Kogotkova O.I.¹, Efremenko V.I.¹, Evchenko N.I.¹, Kulichenko A.N.¹
Affiliations:
1. Stavropol Institute for Plague Control
Issue: Vol 93, No 1 (2016)
Pages: 29-34
Section: ORIGINAL RESEARCHES
URL: https://microbiol.crie.ru/jour/article/view/6
DOI: https://doi.org/10.36233/0372-9311-2016-1-29-34
ID: 6

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Abstract

Aim. Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar. Materials and methods. Vaccine strains: Brucella melitensis Rev-1 and Brucella abortus 19BA. Protein profiling in linear mode on Microflex «Bruker Daltonics» MALDI-TOF mass-spectrometer. Results. A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used. Conclusion. Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.

Keywords

mass-spectrometry, protein profiling, brucellosis causative agent

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FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA

Full Text

Abstract

Keywords

About the authors

D. V. Ulshina

D. A. Kovalev

A. M. Zhirov

N. V. Zharinova

A. A. Khudoleev

O. I. Kogotkova

V. I. Efremenko

N. I. Evchenko

A. N. Kulichenko

References

Supplementary files

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