FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA

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Abstract

Aim. Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar. Materials and methods. Vaccine strains: Brucella melitensis Rev-1 and Brucella abortus 19BA. Protein profiling in linear mode on Microflex «Bruker Daltonics» MALDI-TOF mass-spectrometer. Results. A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used. Conclusion. Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.

About the authors

D. V. Ulshina

Stavropol Institute for Plague Control

Author for correspondence.
Russian Federation

D. A. Kovalev

Stavropol Institute for Plague Control

Russian Federation

A. M. Zhirov

Stavropol Institute for Plague Control

Russian Federation

N. V. Zharinova

Stavropol Institute for Plague Control

Russian Federation

A. A. Khudoleev

Stavropol Institute for Plague Control

Russian Federation

O. I. Kogotkova

Stavropol Institute for Plague Control

Russian Federation

V. I. Efremenko

Stavropol Institute for Plague Control

Russian Federation

N. I. Evchenko

Stavropol Institute for Plague Control

Russian Federation

A. N. Kulichenko

Stavropol Institute for Plague Control

Russian Federation

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Copyright (c) 2016 Ulshina D.V., Kovalev D.A., Zhirov A.M., Zharinova N.V., Khudoleev A.A., Kogotkova O.I., Efremenko V.I., Evchenko N.I., Kulichenko A.N.

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