CROSS-IMMUNOGENICITY OF VARIOUS BACTERIAL L-ASPARAGINASES

Aim. Evaluate immune response in mice against various L-asparaginases and determine their cross-immunogenicity. Materials and methods. The studies were carried out in C 57Bl 6j line mice. Immunogenicity of L-asparaginases was studied: Escherichia coli type II (recombinant) (Medak, Germany) (ЕсА); Erwinia carotovora type II (ErA); Yersinia pseudotuberculosis type II (YpA); Rhodospirillum rubrum type I (RrA); Wollinella succinogenes type II (WsA). Immune response against the administered antigens was determined in EIA. Results. Y. pseudotuberculosis L-asparaginase was the most immunogenic, E. coli - the least immunogenic. E. carotovora, R. rubrum, W. succinogenes asparaginases displayed intermediate immunogenicity. The results of cross-immunogenicity evaluation have established, that blood sera of mice, that had received YpA, showed cross-immunogenicity against all the other L-asparaginase preparations except E. carotovora. During immunization with E. coli L-asparaginase the developed antibodies also bound preparation from E. carotovora. Sera from mice immunized with W. succinogenes, E. carotovora and R. rubrum L-asparaginases had cross-reaction only with EcA and did not react with other preparations. Conclusion. Cross-immunogenicity of the studied L-asparaginases was determined. A sequence of administration of the studied preparation is proposed that allows to minimize L-asparaginase neutralization by cross-reacting antibodies.

Escherichia coli, Erwinia carotovora, Yfersinia pseudotuberculosis, Rhodospirillum rubrum, Wollinella succinogenes, lymphoblastic leucosis, L-asparaginase, Escherichia coli, Erwinia carotovora, Yersinia pseudotuberculosis, Rhodospirillum rubrum, Wollinella succinogenes, EIA, cross-immunogenicity