RAMAN SPECTROSCOPY IN MICROBIOLOGICAL DIAGNOSTICS: ACHIEVEMENTS, ADVANTAGES, PERSPECTIVES


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Abstract

Despite all the diversity of existing methods of detection of microorganisms the question of accelerated diagnostics of causative agents of severe diseases, nosocomial infections and other microbial agents that have epidemiologic significance remains unsolved. In the cases when the result of the study must be available as soon as possible, methods that do not require special prolonged preparation of the studied material and isolation of pure culture of microorganisms are necessary. These include optical-spectral methods, among those combinative light scattering spectroscopy or Raman spectroscopy deserves the most attention, the review being dedicated to its application. The ability to obtain information on components comprising the studied sample within several minute allows to use Raman spectroscopy in many fields of biology and medicine. High specificity of the method is based on absolute uniqueness of specters of various substances and in practice is 96-97%, sensitivity - 95%. Full automation of the process, use of the newest mathematical apparatus for readout and provision of the results of the study allow to avoid the effect of human factor and increase the objectivity of the data obtained. Analytical reliability, timely reception of the result and economical effectiveness entitle to consider Raman spectrometry as a perspective universal express method in microbiological diagnostics.

About the authors

A. V Naumik

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

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