IMMUNOGENIC ACTIVITY OF A CONJUGATE OF SYNTHETIC HEXASACCHARIDE RELATED TO A STREPTOCOCCUS PNEUMONIAE SEROTYPE 14 CAPSULE POLYSACCHARIDE CHAIN FRAGMENT


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Abstract

Aim. Study antigenic and immunogenic activity of a conjugate of synthetic hexasaccharide related to a S. pneumoniae serotype 14 capsule polysaccharide chain fragment with bovine serum albumin (BSA). Materials and methods. Synthetic glucoconjugate based on BSA protein carrier and hexasaccharide ligand reflecting capsule polysaccharide chain fragment was obtained by using squarate method. Natural polysaccharide-protein complex from S. pneumoniae serotype 14 strain was obtained from cultural fluid supernatant by acetone precipitation. IgG titer against hexasaccharide/capsule polysaccharide was determined in antimicrobial sera and sera of mice immunized with glucoconjugate by EIA method. Results. Immunogenic activity of glucoconjugate based on BSA protein carriers and synthetic hexasaccharide reflecting S. pneumoniae serotype 14 capsule protein chain fragment was established. After 2 immunizations antibodies against hexasaccharide ligand and BSA were determined in blood sera of mice. Antibody titers against hexasaccharide exceeded the level in intact mice by 4.2 times. BSA in the conjugate did not have effect on production of antibodies against hexasaccharide. Conclusion. The developed experimental test-system based on synthetic glucoconjugate is useful for evaluation of level of antibodies against S. pneumoniae serotype 14 in infected and, probably, carriers of bacteria.

About the authors

E. A Kurbatova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

D. S Vorobiev

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. B Egorova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

I. B Semenova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

E. V Sukhova

Zelinsky Research Institute of Organic Chemistry, Moscow, Russia

D. V Yashunsky

Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia

Yu. E Tsvetkov

Zelinsky Research Institute of Organic Chemistry, Moscow, Russia

N. E Nifantiev

Zelinsky Research Institute of Organic Chemistry, Moscow, Russia

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Copyright (c) 2013 Kurbatova E.A., Vorobiev D.S., Egorova N.B., Semenova I.B., Sukhova E.V., Yashunsky D.V., Tsvetkov Y.E., Nifantiev N.E.

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