PRESENCE OF YERSINIA ENTEROCOLITICA IN CONDITIONS OF AGRO COMPLEX


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Abstract

Aim. Detection of duration of existence of Yersinia enterocolitica in substrates of agro complex and formation of biofilms by causative agent during artificial semination of forage and meat products. Materials and methods. Y. enterocolitica O9 strain and its rifampicin-resistant (Rmr) mutant were used. Microbial landscape of samples was studied by seeding on selective media (HiMedia), biochemical properties of isolates were controlled on API test-systems (Bio-Merieux). The presence of yopA gene localized on virulence plasmid pCad was determined in PCR. Vital staining of biofilms was carried out by Live/Dead stain (Invitrogen, USA). Visualization of the data was registered by using GMS-510 (USA) microscope with digital camera and Skope Photo software (USA). Formation of yersinia bacterial biofilms was confirmed by using scanning electron microscope (SEM) JSM6380 (Japan). Results. Prolonged duration of existence of Y. enterocolitica in substrates of agro complex with conservation of pCad virulence plasmid by causative agent was detected. SEM demonstrated stages of biofilm formation during artificial semination of animal forage, meat products and materials of food equipment in a wide range of temperatures from 10 to 30° C, and vital stain detected viable yersinia in mature biofilms. Conclusion. Agro complexes are a variant of technogenic foci where ecological conditions for prolonged existence of saprono-sis are formed.

About the authors

M. A Yurova

Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia

V. I Pushkareva

Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia

V. Yu Polyakov

Belozersky Research Institute of Physical-Chemical Biology, Moscow, Russia

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Copyright (c) 2013 Yurova M.A., Pushkareva V.I., Polyakov V.Y.

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