OPTIMIZATION OF CONDITIONS OF QUANTITATIVE EVALUATION OF ARGENTINE HEMORRHAGIC FEVER CAUSATIVE AGENT


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Aim. Optimization of conditions of quantitative evaluation of Argentine hemorrhagic fever causative agent. Materials and methods. Junin virus (XJ P37 strain) was obtained from National collection of viral hemorrhagic fever causative agents of the 1st pathogenicity group of the 33 rd Central Research Testing Institute. Junin virus (XJ P37 strain) culture with biological activity of 5.2 lg PFUxml -1 was used in the experiments. Vero B, 6619-1(D) and GMK-AH-1(D) were obtained from collection of cell culture of the Research Scientific Centre of the 33rd Central Research Testing Institute. Calculation of biological activity of Junin virus during titration in cell cultures was carried out by Kerber method with modification by I.P. Ashmarin. Results. During incubation for 4 - 7 days after the infection of cell monolayer the determined biological activity was 4.4 - 6.4 lg PFUxml -1; the size of the formed negative colonies - (1.5+0.5) mm. Conclusion. The conditions of quantitative evaluation of Argentine hemorrhagic fever were optimized by negative colonies method (using 5 - 7 day Vero B cell culture monolayer with staining of monolayer on day 5 of secondary incubation, recording of results at day 7 after the infection).

About the authors

S. I Syromyatnikova

Research Scientific Centre of the 33rd Central Research Testing Institute of the Ministry of Defense of the Russian Federation, Sergiev Posad, Moscow Region, Russia

V. B Pantyukhov

Research Scientific Centre of the 33rd Central Research Testing Institute of the Ministry of Defense of the Russian Federation, Sergiev Posad, Moscow Region, Russia

I. V Shatokhina

Research Scientific Centre of the 33rd Central Research Testing Institute of the Ministry of Defense of the Russian Federation, Sergiev Posad, Moscow Region, Russia

V. A Markin

Research Scientific Centre of the 33rd Central Research Testing Institute of the Ministry of Defense of the Russian Federation, Sergiev Posad, Moscow Region, Russia

A. P Pirozhkov

Research Scientific Centre of the 33rd Central Research Testing Institute of the Ministry of Defense of the Russian Federation, Sergiev Posad, Moscow Region, Russia

M. A Timofeev

Research Scientific Centre of the 33rd Central Research Testing Institute of the Ministry of Defense of the Russian Federation, Sergiev Posad, Moscow Region, Russia

T. E Sizikova

Research Scientific Centre of the 33rd Central Research Testing Institute of the Ministry of Defense of the Russian Federation, Sergiev Posad, Moscow Region, Russia

I. G Rymyantseva

Research Scientific Centre of the 33rd Central Research Testing Institute of the Ministry of Defense of the Russian Federation, Sergiev Posad, Moscow Region, Russia

S. V Borisevich

Research Scientific Centre of the 33rd Central Research Testing Institute of the Ministry of Defense of the Russian Federation, Sergiev Posad, Moscow Region, Russia

References

  1. Ашмарин И.П., Воробьев А.А. Статистические методы в микробиологических исследованиях. Л., Гос. изд. мед. лит., 1962.
  2. Маркин В.А., Пантюхов В.Б., Марков В.И., Бондарев В.П. Аргентинская геморрагическая лихорадка. Журн. микробиол. 2012, 2: 78-87.
  3. Маркин В.А., Марков В.И. Вирусные геморрагические лихорадки - эволюция эпидемического потенциала. Журн. микробиол. 2002, 2: 91-98.
  4. Мельникова О.В., Титенко А.М., Андаев Е.И. Санитарная охрана территории от завоза и распространения особо опасных вирусных инфекций. Аргентинская и Боливийская геморрагические лихорадки. Пробл. особо опасных инф. 2010, 104 (2): 29-34.
  5. Ambrossio A.M., Enria D.A. Junin virus isolation from lymphomononuclear cells of patients wich Argentine hemorrhagic fever. Intervirology. 1986, 25 (20): 97-102.
  6. Bushar G., Sagripanti J.L. Method for improving accuracy of virus titration: Standardization of plague assay for Junin virus. J. Virol. Meth. 1990, 30: 99-108.
  7. Lascano E.F., Birria M.L. Diagnosis of Junin virus in cell cultures by immunoperoxi-dase staining. Arch. Virol. 1981, 70 (1): 79-82.
  8. Viral hemorrhagic fevers: Current, comprehensive information on pathogenesis, microbiology, epidemiology, diagnosis, treatment and prophylaxis. CDC: 2004 CTDRAP / JDSA. URL: http://www.idsociety.org. (дата обращения: 20.05.12).

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Copyright (c) 2013 Syromyatnikova S.I., Pantyukhov V.B., Shatokhina I.V., Markin V.A., Pirozhkov A.P., Timofeev M.A., Sizikova T.E., Rymyantseva I.G., Borisevich S.V.

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