MOLECULAR-GENETIC CHARACTERISTIC OF ASTROVIRUSES CIRCULATING IN NIZHNYNOVGOROD


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Abstract

Aim. Genotyping of astroviruses isolated from children with acute enteric infection (AEI) on the territory
of Nizhny Novgorod in 2006 - 2011. Materials and methods. Samples of feces from children hospitalized in
intestine infection department of a Nizhny Novgorod infectious diseases hospitals served as study material.
Astroviruses were detected by reverse transcription polymerase chain reaction method. Astrovirus genotypes
were determined based on phylogenetic analysis of nucleotide sequence of genome fragment coding capsid
protein. Results. During examination of 5759 children with AEI from July 2006 to June 2011 astroviruses
were detected in 2.6% of cases, and in 2010 - 2011 the frequency of astrovirus detection (5.19%) was significantly
higher than in the previous epidemic seasons and the average based on the whole observation period.
Genotyping of the detected astroviruses showed that genotype 1, 2, 3, 4, 5 astroviruses circulated on the
territory of Nizhny Novgorod with predomination of genotype 1. Genotype 1 astroviruses are presented by
genetic lineages 1a, 1b, 1d with predomination of lineage 1a. From the start of 2010 all the detected isolates
from genetic lineage 1a belonged to a single new sublineage - 1a-2010. The second by quantity of detected
isolates were genotype 5 astroviruses identified for the first time in Nizhny Novgorod in July 2010. Genotype
2, 3 and 4 astroviruses were detected in isolated cases. Conclusion. Activation of astrovirus circulation in the
population of Nizhny Novgorod that was shown by a significant increase of frequency of their detection in
children with AEI in 2010 - 2011 epidemic season with the highest probability was caused by appearance of
genotype 5 astroviruses, that had not previously been detected in this territory and other territories of Russian
Federation.

About the authors

N V Epifanova

N A Novikova

E I Efimov

O V Parfenova

L B Lukovnikova

S G Fomina

N V Epifanova

Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Russia

Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Russia

N A Novikova

Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Russia

Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Russia

E I Efimov

Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Russia

Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Russia

O V Parfenova

Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Russia

Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Russia

L B Lukovnikova

Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Russia

Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Russia

S G Fomina

Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Russia

Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Russia

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Copyright (c) 2012 Epifanova N.V., Novikova N.A., Efimov E.I., Parfenova O.V., Lukovnikova L.B., Fomina S.G., Epifanova N.V., Novikova N.A., Efimov E.I., Parfenova O.V., Lukovnikova L.B., Fomina S.G.

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