VLIYaNIE AKTIVNYKh FORM KISLORODA NA ADGEZIVNYE KhARAKTERISTIKI I PRODUKTsIYu BIOPLENOK BAKTERIYaMI

Aim. Study of influence of low concentrations of active forms of oxygen on the ability of bacteria
to form biofilms and their adhesive characteristics. Materials and methods. Modification of
the studied properties of bacteria was performed by treatment of the bacteria with hydrogen peroxide
or hydroxyl radicals at concentrations of 0.5 mM that causes death of 5 - 7% of cells and
non-lethal - 0.05 and 0.005 mM. Adhesive characteristics of Escherichia coli, Staphylococcus
aureus, Bacillus subtilis and Klebsiella pneumoniae bacteria were determined by using human
erythrocytes. The degree of biofilm formation in plates modified by collagen from human fibroblasts
was determined by the amount of bound crystal violet. Results. A significant decrease (7 -
17%) of the adhesion of E. coli, S. aureus, K. pneumoniae to erythrocytes under the influence of
hydrogen peroxide and hydroxyl radicals at the concentrations of 0.5 and 0.05 mM when compared
with the control was detected. 11 - 40% stimulation of E. coli, S. aureus, K. pneumoniae biofilm
formation by active forms of oxygen in all the used concentrations with simultaneous insignificant
biomass growth increase was noted. Active forms of oxygen did not influence significantly the
studied properties of B. subtilis. Conclusion. Active oxygen forms have regulatory influence on the
character of interaction of bacteria with surfaces colonized by them, that determines structuralfunctional
state of microbial biocenoses.

active oxygen forms, adhesion, bacteria, biofilms