ENZYME IMMUNOASSAY-BASED ANALYSIS OF OspC RECOMBINANT PROTEINS FROM BORRELIA GARINII AND BORRELIA AFZELII ISOLATED IN WEST SIBERIA


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Abstract

Aim. To study the ability of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia to interact with serum antibodies from patients with tick-borne borreliosis (TBB). Materials and methods. Recombinant antigens OspC B.garinii and OspC B.afzelii , serum samples from patients with TBB were used as well as solid-phase enzyme immunoassay and parametric and non-parametric statistical methods. Results. Higher antigenic activity of B.garinii OspC compared with OspC from B.afzelii was observed when these recombinant proteins were compared in enzyme immunoassay. Detection rate of class M and G immunoglobulins to B.garinii OspC in sera of patients with TBB was 60.5% and 70% respectively. Conclusion. Obtained results indicate high immunoreactivity of OspC recombinant proteins from B.garinii and B.afzelii and point to perspective of their combined use for serological diagnostics of TBB.

About the authors

V. S Karavaev

Research Institute of Biochemistry, Novosibirsk, Russia

A. V Ryabchenko

Research Institute of Biochemistry, Novosibirsk, Russia

A. B Beklemishev

Research Institute of Biochemistry, Novosibirsk, Russia

References

  1. Иванов И.Д., Беклемишев А.Б. Клонирование и анализ экспрессии фрагмента гена flab новосибирского изолята Borrelia garinii NT29 в клетках E. coli и исследование антигенных свойств рекомбинантного белка. Бюлл. СО РАМН. 2002, 1: 97-102.
  2. Рябченко А.В., Беклемишев А.Б. Мониторинг зараженности клещей Ixodes persulcatus Schulze, населяющих рекреационную зону Новосибирского научного центра, возбудителями Лайм-боррелиоза человека. Вестник НГУ. 2006, 12 (3—4): 109-110.
  3. Baranton G., Ras N.M., Postic D. Molecular epidemiology of the aetiological agents of Lyme borreliosis. Klin. Woch. 1998, 110 (24): 850-855.
  4. Bunkis J., Olsen B., Westman G. et al. Variable serum immunoglobulin responses against different Borrelia burgdorferi sensu lato species in population at risk for patients with Lyme disease. J. Clin. Microbiol. 1995, 33 (6): 1473-1478.
  5. Lagal V., Postic D., Ruzic-Sabljic E. J. Genetic diversity among Borrelia strains determined by single-strand conformation polymorphism analysis of the ospC gene and its association with invasiveness. Ibid. 2003, 41: 5059-5065.
  6. Magnarelli L.A, Ijdo J.W, Padula S.J. Serologic diagnosis of lyme borreliosis by using enzymelinked immunosorbent assays with recombinant antigens. Ibid. 2000, 38 (5): 1735-1739.
  7. Pachner A.R., Dail D., Li L. Humoral immune response associated with Lyme borreliosis in nonhuman primates: analysis by immunoblotting and enzyme-linked immunosorbent assay with sonicates or recombinant proteins. Clin. Diagn. Lab. Immunol. 2002, 9 (6): 1348-1355.
  8. Wang G., van Dam A.P. et al. Molecular typing of Borrelia burgdorferi sensu lato: taxonomic, epidemiological, and clinical implications. J. Clin. Microbiol. Rev. 1999, 12: 633-653.

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Copyright (c) 2010 Karavaev V.S., Ryabchenko A.V., Beklemishev A.B.

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