ENZYME IMMUNOASSAY-BASED ANALYSIS OF OspC RECOMBINANT PROTEINS FROM BORRELIA GARINII AND BORRELIA AFZELII ISOLATED IN WEST SIBERIA


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Abstract

Aim. To study the ability of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia to interact with serum antibodies from patients with tick-borne borreliosis (TBB). Materials and methods. Recombinant antigens OspC B.garinii and OspC B.afzelii , serum samples from patients with TBB were used as well as solid-phase enzyme immunoassay and parametric and non-parametric statistical methods. Results. Higher antigenic activity of B.garinii OspC compared with OspC from B.afzelii was observed when these recombinant proteins were compared in enzyme immunoassay. Detection rate of class M and G immunoglobulins to B.garinii OspC in sera of patients with TBB was 60.5% and 70% respectively. Conclusion. Obtained results indicate high immunoreactivity of OspC recombinant proteins from B.garinii and B.afzelii and point to perspective of their combined use for serological diagnostics of TBB.

About the authors

V. S Karavaev

Research Institute of Biochemistry, Novosibirsk, Russia

A. V Ryabchenko

Research Institute of Biochemistry, Novosibirsk, Russia

A. B Beklemishev

Research Institute of Biochemistry, Novosibirsk, Russia

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Copyright (c) 2010 Karavaev V.S., Ryabchenko A.V., Beklemishev A.B.

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