Email this article ENZYME IMMUNOASSAY-BASED ANALYSIS OF OspC RECOMBINANT PROTEINS FROM BORRELIA GARINII AND BORRELIA AFZELII ISOLATED IN WEST SIBERIA
- Authors: Karavaev V.S1, Ryabchenko A.V1, Beklemishev A.B1
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Affiliations:
- Research Institute of Biochemistry, Novosibirsk, Russia
- Issue: Vol 87, No 1 (2010)
- Pages: 20-23
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13396
- ID: 13396
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Abstract
Aim. To study the ability of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia to interact with serum antibodies from patients with tick-borne borreliosis (TBB). Materials and methods. Recombinant antigens OspC B.garinii and OspC B.afzelii , serum samples from patients with TBB were used as well as solid-phase enzyme immunoassay and parametric and non-parametric statistical methods. Results. Higher antigenic activity of B.garinii OspC compared with OspC from B.afzelii was observed when these recombinant proteins were compared in enzyme immunoassay. Detection rate of class M and G immunoglobulins to B.garinii OspC in sera of patients with TBB was 60.5% and 70% respectively. Conclusion. Obtained results indicate high immunoreactivity of OspC recombinant proteins from B.garinii and B.afzelii and point to perspective of their combined use for serological diagnostics of TBB.
About the authors
V. S Karavaev
Research Institute of Biochemistry, Novosibirsk, Russia
A. V Ryabchenko
Research Institute of Biochemistry, Novosibirsk, Russia
A. B Beklemishev
Research Institute of Biochemistry, Novosibirsk, Russia
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