Email this article USE OF STREPTOCOCCUS AGALACTIAE sak0192 GENE POLYMORPHISM AS A MOLECULAR EPIDEMIOLOGIC MARKER
- Authors: Rozhdestvenskaya A.S1, Bhide M.2, I.Mikula J.2, Mikula I.2, Dmitriev A.V1
-
Affiliations:
- Research Institute of Experimental Medicine, SaintPetersburg, Russia
- University of Veterinary Medicine, Koshice, Slovakia
- Issue: Vol 86, No 5 (2009)
- Pages: 38-43
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.10.2009
- URL: https://microbiol.crie.ru/jour/article/view/13293
- ID: 13293
Cite item
Full Text
Abstract
Aim . To study structure of sak0192 gene in S.agalactiae strains isolated from animals and to assess potential for using it as a molecular epidemiologic marker. Materials and methods . One hundred and fourteen strains of S.agalactiae isolated from cow milk were used. Presence of sak0192 gene as well as pathogenicity genes bac, bca, scpB was determined by PCR. Alleles of sak0192 gene were identified by SSCP method, and their nucleotide sequences — by sequencing. Results . In sak0192 gene of S.agalactiae strains direct repeats and spacers were revealed as well as heterogeneity in its nucleotide sequence. Eight different alleles of sak0192 gene were identified in 114 strains, five of which were characteristic only for strains isolated from animals. Complex analysis of sak0192 gene allele and presence of bac, bca, and scpB revealed 16 different genetic variants, only 3 of which were characteristic for strains isolated from animals and from humans. Conclusion . Polymorphism of sak0192 gene could be used for diagnostics and differentiation of S.agalactiae strains and, together with combination of pathogenicity genes bac, bca, and scpB, for identification of epidemically significant clones — agents of infectious disease in humans and animals.
Full Text
ИСПОЛЬЗОВАНИЕ ПОЛИМОРФИЗМА ГЕНА sak0192 STREPTOCOCCUS AGALACTIAE В КАЧЕСТВЕ МОЛЕКУЛЯРНОЭПИДЕМИОЛОГИЧЕСКОГО МАРКЕРА×
About the authors
A. S Rozhdestvenskaya
Research Institute of Experimental Medicine, SaintPetersburg, Russia
M. Bhide
University of Veterinary Medicine, Koshice, Slovakia
Jr. I.Mikula
University of Veterinary Medicine, Koshice, Slovakia
I. Mikula
University of Veterinary Medicine, Koshice, Slovakia
A. V Dmitriev
Research Institute of Experimental Medicine, SaintPetersburg, Russia
References
- Дмитриев А.В., Шен А.Д., Тотолян А.А. Ген sak0192 Streptococcus agalactiae содержит прямые повторы и спейсеры — генетические маркеры для характеристики штаммов. Мол. ген. микробиол. вирусол. 2007, 4: 37-41.
- Bassam B.J., Caetano-Anollés G., Gresshoff P.M. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 1991, 196: 80-83.
- Bohnsack J.F., Whiting. A., Gottschalk M. et al. The population structure of invasive and colonizing strains of Streptococcus agalactiae from neonates of six U.S. academic centers from 1995 to 1999. J. Clin. Microbiol. 2008, 46: 1285-1291.
- Dmitriev A., Shakleina E., Tkacikova L. et al. Genetic heterogeneity of the pathogenic potentials of human and bovine group B streptococci. Folia Microbiol. 2002, 47: 291-295.
- Dmitriev.A., Tkacikova L., Suvorov A. et al. Comparative genetic study of group B streptococcal strains of human and bovine origin. Ibid. 1999, 44: 449-453.
- Dmitriev A., Totolian A., Tkacikova L. et al. Features of the genome structure of group B streptococci of bovine origin. Folia Veterinaria. 2001, 45: 57-63.
- Duarte R.S., Bellei B.C., Miranda O.P. et al. Distribution of antimicrobial resistance and virulence-related genes among Brazilian group B streptococci recovered from bovine and human sources. Antimicrob. Agents Chemother. 2005, 49: 97-103.
- Hery-Arnaud G., Bruant G., Lanotte P. et al. Mobile genetic elements provide evidence for a bovine origin of clonal complex 17 of Streptococcus agalactiae. Appl. Environ. Microbiol. 2007, 73: 4668-4672.
- Jensen N.E., Aarestrup F.M. Epidemiological aspects of group B streptococci of bovine and human origin. Epidemiol. Infect. 1996, 117: 417-422.
- Keefe G.P. Can. Streptococcus agalactiae mastitis: a review. Vet. J. 1997, 38: 429-437.
- Luan S., Granlund M., Sellin M. et al. Multilocus sequence typing of Swedish invasive group B Streptococcus isolates indicates a neonatally associated genetic lineage and capsule switching. J. Clin. Microbiol. 2005, 43: 3727-3733.
- Martinez G., Harel J., Higgins R. et al. Characterization of Streptococcus agalactiae isolates of bovine and human origin by randomly amplified polymorphic DNA analysis. Ibid. 2000, 38: 71-78.
- Regan J.A., Klebanoff M.A., Nugent R.P. et al. VIP Study Group. Colonization with group B streptococci in pregnancy adverse outcome. Am. J. Obstet. Gynecol. 1996, 174: 1354-1360.
- Shakleina E., Dmitriev A., Tkacikova L. et al. Presence of IS elements in group B streptococci of bovine origin. Ind. J. Med. Red. 2004, 119 (suppl.): 242-246.
- Tettelin H., Masignani V., Cieslewicz M.J. et al. Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial «pan-genome». Proc. Natl. Acad. Sci. USA. 2005, 102: 13950-13955.
Supplementary files
