FEATURES OF FORMATION OF BACTERIAL BIOFILMS IN CONDITIONS OF SPACE FLIGHT

Cover Page


Cite item

Full Text

Abstract

Aim. Study the effect of microgravitation on the formation of Lactobacillus plantarum 8PA-3 bacterial biofilms in the conditions of space flight. Materials and methods. Information on the effect of microgravitation on the development of biofilms was obtained during study of L. plantarum 8PA-3 probiotic lactobacilli in special equipment in the process of execution of space experiments in the Russian segment of the International Space Station. Comparative analysis of growth of plankton and biofilm forms of cells developing in the conditions of space flight and surface conditions was carried out by microbiologic and electron-microscopy methods using scanning and transmission electron microscopy. Results. Accelerated dynamics of formation of L. plantarum 8PA-3 lactobacilli biofilm on the surface of polymer substrate was shown for the first time during the space experiment. Microbiological analysis of the bacterial culture has also confirmed the accelerated growth of L. plantarum 8PA-3 under microgravitation compared with surface conditions. Ultrastructure of plankton form of L. plantarum 8PA-3 taking part in formation of biofilms in conditions of microgravitation was detected for the first time in the space experiment. Conclusion. Data on comparative electron-microscopic analysis obtained in space experiments are important for scientific justification of the effect of microgravitation on bacterial communities developing as biofilms - the most natural form of existence of microorganisms. The results obtained could be taken into consideration during creation of novel antibacterial means and disinfectants as well as methods of treatment of surfaces of modules of piloted space complexes that could allow to clarify methods of effective prophylaxis of biofilm spread which pose a risk of health of the crew and normal functioning of equipment in the International Space Station.

About the authors

O. V. Rybalchenko

St. Petersburg State University; State Research Institute of Highly Pure Biopreparations

Author for correspondence.
Russian Federation

O. G. Orlova

St. Petersburg State University; State Research Institute of Highly Pure Biopreparations

Russian Federation

O. N. Vishnevskaya

St. Petersburg State University; State Research Institute of Highly Pure Biopreparations

Russian Federation

V. V. Kapustina

St. Petersburg State University

Russian Federation

I. L. Potokin

State Research Institute of Highly Pure Biopreparations

Russian Federation

V. V. Lavrikova

«Biopreparat»

Russian Federation

References

  1. Андреев-Андриевский А. А., Шенкман Б. С., Попова А. С. и др. Экспериментальные исследования на мышах по программе полета биоспутника «Бион-М 1». Авиакосм. экол. мед. 2014, 48 (1): 14-27.
  2. Бондаренко В.М. Роль условно-патогенных бактерий при хронических воспалительных процессах различной локализации. М., Триада, 2011.
  3. Григоренко Д. Е., Сапин М. Р. Перестройка лимфоидных структур селезенки у песчанок после космического полета. Морфология. 2012, 142 (4): 67-71.
  4. Кобатов А.И.,Вербицкая Н.Б.,Добролеж О.В., Рыбальченко О.В.,Петров Л.Н.Изучение пробиотических характеристик Lactobacillus acidophilus, выращенных в условиях космического полета. Медицина экстремальных ситуаций. 2008, 4 (26): 66- 78.
  5. Пономарев С. А., Антропова Е. Н., Берендеева Т. А. и др. Особенности изменений показателей врожденного иммунитета при воздействии на организм человека неблагоприятных факторов длительного космического полета. Авиакосм. экол. мед. 2013, 47 (4): 123-124.
  6. Потапов А. Н., Синяк Ю. Е., Петров В. М. Проблемы медико-биологического обеспечения межпланетных экспедиций. Авиакосм. экол. мед. 2013, 47 (1): 55-60.
  7. Рыбальченко О.В. Электронно-микроскопическое исследование межклеточных взаимодействий микроорганизмов при антагонистическом характере взаимоотношений. Микробиология. 2006, 75 (4): 550-555.
  8. Рыбальченко О.В., Бондаренко В.М., Гуслева О.Р. и др. Дезорганизация биопленок клинических штаммов стафилококков метаболитами лактобацилл. Журн. микробиол. 2010, 6: 66-70.
  9. Branda S.S., Vik A., Friedman L., Kolter R. Biofilms: the matrix revised. Trends Microbial. 2005, 13:21-25.
  10. Donald R.M., Costerton J.W. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin. Microbiol. Rev. 2002, 15: 167-193.
  11. Macfarlane S. Microbial biofilm communities in the gastrointestinal tract. J. Clin. Gastroenterol. 2008, 242 (3): S142-S143.
  12. Miller M.B., Bassler B.L. Quorum sensing in bacteria. Ann. Rev. Microbiol. 2001, 55: 165-199.
  13. Novikova N.D. Review of the knowledge of microbial contamination of the russian manned spacecraft. Microb. Ecol. 2004, 47: 127-132.
  14. Olson M.E., Ceri H., Morck D.W. et al. Biofilm bacteria: formation and comparative susceptibility to antibiotics. Can. J. Vet. Res. 2002, 66: 86-92.
  15. Popat R., Crusz S., Doggie S. The social behaviours ofbacterial pathogens. Brit. Med. Bullet. 2008, 87: 63-75.
  16. Pratt L.A., Kolter R. Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type.I pili. Mol. Microbiol. 1998, 30: 285-294.
  17. Rybalchenko O., Bondarenko V., Rozlomiy V., Orlova O. Ultrastructural organization of biofilms of opportunistic microorganisms - representatives of gut human microbiota. Genes Nutrition. 2010,1.5: S92.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2016 Rybalchenko O.V., Orlova O.G., Vishnevskaya O.N., Kapustina V.V., Potokin I.L., Lavrikova V.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС77-75442 от 01.04.2019 г.