Email this article HBV COVALENTLY CLOSED CIRCULAR DNA AS A MARKER OF PREVALENCE OF OCCULT HEPATITIS В IN PATIENTS WITH HBV, HDV AND HCV INFECTION IN UZBEKISTAN
- Authors: Semenov A.V.1,2,3, Ostankova Y.V.1, Faizullaev K.N.4, Kazakova E.I.4, Kozlov A.V.3, Musabaev E.I.4, Totolyan A.A.1,2
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Affiliations:
- Pasteur Research Institute of Epidemiology and Microbiology
- Pavlov State Medical University
- Mechnikov North-Western State Medical University
- Research Institute of Virology
- Issue: Vol 93, No 5 (2016)
- Pages: 43-49
- Section: ORIGINAL RESEARCHES
- Submitted: 10.04.2019
- Published: 28.10.2016
- URL: https://microbiol.crie.ru/jour/article/view/86
- DOI: https://doi.org/10.36233/0372-9311-2016-5-43-49
- ID: 86
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Abstract
Aim. Evaluate significance of covalently closed circular DNA of hepatitis В virus as a marker for detection of occult viral hepatitis В in Uzbekistan population with hepatitis of various genesis. Materials and methods. Blood plasma and liver biopsy from 39 patients with different severity levels of liver fibrosis and cirrhosis served as study material. HBV covalently closed circular DNA detection was carried out according to Pollicino T. et al. (2004). Results. Covalently closed circular DNA of hepatitis В virus was detected in 82% of samples, including in 54.5% of patients with chronic viral hepatitis C (CVHC) and in 100% of patients with hepatitis of unknown etiology. Quantitative evaluation of content of covalently closed circular DNA of hepatitis В virus in liver tissue in patients with CVHB has shown an average of 2.5 copies of HBV genome as ccc DNA per cell, in patients with CVHB + D an average of 0.7 copies/cell, in patients with co-infection by HCV and HBV - 0.5 copies/cell, in patients with CVHC an average of 0.12 copies/cell, and in patients with cryptogenic hepatitis - 0.2 copies/cell. Conclusion. Detection of HBV DNA is a complex problem for effective laboratory diagnostics of hepatitis. Detection of H BV ccc DNA as a marker of occult hepatitis В in patients with CVHC and patients with hepatitis of unclear etiology is an important factor for diagnostics, selection of adequate therapy, prognosis of disease outcome and prevention of development of severe liver diseases.
About the authors
A. V. Semenov
Pasteur Research Institute of Epidemiology and Microbiology; Pavlov State Medical University; Mechnikov North-Western State Medical University
Author for correspondence.
Email: noemail@neicon.ru
Россия
Yu. V. Ostankova
Pasteur Research Institute of Epidemiology and Microbiology
Email: noemail@neicon.ru
Россия
Kh. N. Faizullaev
Research Institute of Virology
Email: noemail@neicon.ru
Россия
E. I. Kazakova
Research Institute of Virology
Email: noemail@neicon.ru
Россия
A. V. Kozlov
Mechnikov North-Western State Medical University
Email: noemail@neicon.ru
Россия
E. I. Musabaev
Research Institute of Virology
Email: noemail@neicon.ru
Россия
A. A. Totolyan
Pasteur Research Institute of Epidemiology and Microbiology; Pavlov State Medical University
Email: noemail@neicon.ru
Россия
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