Email this article HBV COVALENTLY CLOSED CIRCULAR DNA AS A MARKER OF PREVALENCE OF OCCULT HEPATITIS В IN PATIENTS WITH HBV, HDV AND HCV INFECTION IN UZBEKISTAN
- Authors: Semenov A.V.1,2,3, Ostankova Y.V.1, Faizullaev K.N.4, Kazakova E.I.4, Kozlov A.V.3, Musabaev E.I.4, Totolyan A.A.1,2
-
Affiliations:
- Pasteur Research Institute of Epidemiology and Microbiology
- Pavlov State Medical University
- Mechnikov North-Western State Medical University
- Research Institute of Virology
- Issue: Vol 93, No 5 (2016)
- Pages: 43-49
- Section: ORIGINAL RESEARCHES
- URL: https://microbiol.crie.ru/jour/article/view/86
- DOI: https://doi.org/10.36233/0372-9311-2016-5-43-49
- ID: 86
Cite item
Full Text
Abstract
Aim. Evaluate significance of covalently closed circular DNA of hepatitis В virus as a marker for detection of occult viral hepatitis В in Uzbekistan population with hepatitis of various genesis. Materials and methods. Blood plasma and liver biopsy from 39 patients with different severity levels of liver fibrosis and cirrhosis served as study material. HBV covalently closed circular DNA detection was carried out according to Pollicino T. et al. (2004). Results. Covalently closed circular DNA of hepatitis В virus was detected in 82% of samples, including in 54.5% of patients with chronic viral hepatitis C (CVHC) and in 100% of patients with hepatitis of unknown etiology. Quantitative evaluation of content of covalently closed circular DNA of hepatitis В virus in liver tissue in patients with CVHB has shown an average of 2.5 copies of HBV genome as ccc DNA per cell, in patients with CVHB + D an average of 0.7 copies/cell, in patients with co-infection by HCV and HBV - 0.5 copies/cell, in patients with CVHC an average of 0.12 copies/cell, and in patients with cryptogenic hepatitis - 0.2 copies/cell. Conclusion. Detection of HBV DNA is a complex problem for effective laboratory diagnostics of hepatitis. Detection of H BV ccc DNA as a marker of occult hepatitis В in patients with CVHC and patients with hepatitis of unclear etiology is an important factor for diagnostics, selection of adequate therapy, prognosis of disease outcome and prevention of development of severe liver diseases.
About the authors
A. V. Semenov
Pasteur Research Institute of Epidemiology and Microbiology; Pavlov State Medical University; Mechnikov North-Western State Medical University
Author for correspondence.
Email: noemail@neicon.ru
Russian Federation
Yu. V. Ostankova
Pasteur Research Institute of Epidemiology and Microbiology
Email: noemail@neicon.ru
Russian Federation
Kh. N. Faizullaev
Research Institute of Virology
Email: noemail@neicon.ru
Russian Federation
E. I. Kazakova
Research Institute of Virology
Email: noemail@neicon.ru
Russian Federation
A. V. Kozlov
Mechnikov North-Western State Medical University
Email: noemail@neicon.ru
Russian Federation
E. I. Musabaev
Research Institute of Virology
Email: noemail@neicon.ru
Russian Federation
A. A. Totolyan
Pasteur Research Institute of Epidemiology and Microbiology; Pavlov State Medical University
Email: noemail@neicon.ru
Russian Federation
References
- Габдрахманов И.А., Семенов А.В., Останкова Ю.В. идр. Взаимосвязи вирусологических и морфологических показателей в фазах иммунного контроля и реактивации у больных хроническим гепатитом В. Журнал инфектологии. 2015, 7 (4): 37-43.
- Семенов А.В., Власова И.А., Останкова Ю. В., Тотолян Арег А. Количественное определение HBsAg в сыворотке крови и кольцевой ковалентно замкнутой ДНК вируса гепатита В в ткани печени как маркеры активности хронического вирусного гепатита В. Журн. микробиол. 2014, 1: 55-61.
- Alghamdi A., Aref N., El-Hazmi М. et al. Correlation between hepatitis В surface antigen titers and HBV DNA levels. Saudi J. Gastroenterol. 2013, 19 (6): 252-257.
- Branco F., Mattos A.A., Coral G.P. et al. Occult hepatitis В virus infection in patients with chronic liver disease due to hepatitis C virus and hepatocellular carcinoma in Brazil. Arq. Gastroenterol. 2007, 44 (1): 58-63.
- Fukuda R., Ishimura N., Niigaki M. et al. Serologically silent hepatitis В virus coinfection in patients with hepatitis C virus-associated chronic liver disease: clinical and virological significance. J. Med. Virol. 1999, 58: 201-207.
- Georgiadou S.P., Zachou K., Liaskos C. et al. Occult hepatitis В virus infection in patients with autoimmune liver diseases. Liver Int. 2009, 29 (3): 434-442.
- Guo J.T., Guo H. Metabolism and function of hepatitis В virus cccDNA: Implications for the development of cccDNA-targeting antiviral therapeutics. Antiviral Research. 2015, 122: 91-100.
- Huqng X., Hollinger F.B. Occult hepatitis В virus infection and hepatocellular carcinoma: a systematic review. J. Viral Hepat. 2014, 21: 153-162.
- Lozano R., Naghavi M., Foreman K. et al. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis forthe Global Burden of Disease Study 2010. Lancet. 2012, 380: 2095-2128.
- Pollicino T., Squadrito G., Cerenzia G. et al. Hepatitis В virus maintains its pro-oncogenic properties in the case of occult HBV infection. Gastroenterology. 2004, 126 (1): 102-110.
- Raimondo G., Pollicino T., Cacciola I. et al. Occult hepatitis В virus infection. J. Hepatol. 2007,46: 160-170.
- Ramezani A., Banifazl M., Eslamifar A. et al. Occult hepatitis В infection in different high risk patients. Hepat. Mon. 2012, 12: 467-468.
- Rizvi M., Azam M., Sultan A. et al. Prevalence of genotype D in chronic liver disease patients with occult HBV infection in northern region of India. Indian J. Pathol. Microbiol. 2014, 57 (4): 537-541. •
- Rodriguez-Inigo E., Bartolome J., Ortiz-Movilla N. et al. Hepatitis C virus (HCV) and hepatitis В virus (HBV) can coinfect the same hepatocyte in the liver of patients with chronic HCV and occult HBV infection. J. Virol. 2005, 79 (24): 15578-15581.
- Ruzibakiev R., Kato H., Ueda R. et al. Risk factors and seroprevalence of hepatitis В virus, hepatitis C virus, and human immunodeficiency virus infection in Uzbekistan. Intervirology. 2001,44 (6): 327-332.
- Squadrito G., Cacciola I., Alibrandi A. et al. Impact of occult hepatitis В virus infection on the outcome of chronic hepatitis C. J. Hepatol. 2013, 59 (4): 696-700.
- Torbenson M., Thomas D.L. Occult hepatitis B. Lancet Infect. Dis. 2002, 2: 479-486.
- Wu Z.F., Xu Z., Li W.S. et al. Impact of occult hepatitis В virus infection on outcome after resection fornon-B non-C hepatocellular carcinoma. J. Surg. Res. 2015, 193 (1): 153-160.
Supplementary files
