Email this article MOLECULAR-BIOLOGICAL MARKERS OF HEPATITIS В IN PATIENTS WITH LIVER FIBROSIS/CIRRHOSIS IN UZBEKISTAN
- Authors: Ostankova Y.V.1, Semenov A.V.1,2,3, Faizullaev K.N.4, Kazakova E.I.4, Kozlov A.V.3, Musabaev E.I.4, Totolyan A.A.1,2
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Affiliations:
- Pasteur Research Institute of Epidemiology and Microbiology
- Pavlov State Medical University
- Mechnikov North-Western State Medical University
- Research Institute of Virology
- Issue: Vol 93, No 5 (2016)
- Pages: 34-43
- Section: ORIGINAL RESEARCHES
- Submitted: 10.04.2019
- Published: 28.10.2016
- URL: https://microbiol.crie.ru/jour/article/view/85
- DOI: https://doi.org/10.36233/0372-9311-2016-5-34-43
- ID: 85
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Abstract
Aim. Evaluate prevalence of genetic variants of hepatitis В viruses in population of various regions of Uzbekistan with hepatitis of various genesis and different severity levels of liver fibrosis and cirrhosis. Materials and methods. Blood plasma and liver biopsy from 39 patients with different severity levels of liver fibrosis and cirrhosis served as study material. Genotyping based on direct sequencing of Pre-Sl/Pre-S2/S HBV DNA region was applied. Results. Hepatitis В virus was detected in 32 samples ofthe 39 provided, frequency of occurrence - 82%, respectively. Phylogenetic analysis has shown, that only genotype D was detected among the examined patients, hepatitis В virus subtype D1 predominated (84.38%) compared with D2 (3.12%) and D3 (12.5%) subtypes. Conclusion. Large-scale sequencing of HBV in Central Asia will allow to evaluate routes of transmission and time of evolutionary separation of virus isolates. Understanding the epidemiology of the infectious process is important for development of programs for prophylaxis and therapy of the infection.
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About the authors
Yu. V. Ostankova
Pasteur Research Institute of Epidemiology and Microbiology
Author for correspondence.
Email: noemail@neicon.ru
Россия
A. V. Semenov
Pasteur Research Institute of Epidemiology and Microbiology; Pavlov State Medical University; Mechnikov North-Western State Medical University
Email: noemail@neicon.ru
Россия
Kh. N. Faizullaev
Research Institute of Virology
Email: noemail@neicon.ru
Россия
E. I. Kazakova
Research Institute of Virology
Email: noemail@neicon.ru
Россия
A. V. Kozlov
Mechnikov North-Western State Medical University
Email: noemail@neicon.ru
Россия
E. I. Musabaev
Research Institute of Virology
Email: noemail@neicon.ru
Россия
A. A. Totolyan
Pasteur Research Institute of Epidemiology and Microbiology; Pavlov State Medical University
Email: noemail@neicon.ru
Россия
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