Email this article DETECTION OF DEGREE OF MICROECOLOGICAL DISTURBANCE OF INTESTINE BASED ON CALCULATION OF LOCAL ANTIOXIDANT INDEX
- Authors: Gapon M.N.1, Ternovskaya L.N.1
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Affiliations:
- Rostov Research Institute of Microbiology and Parasitology
- Issue: Vol 93, No 2 (2016)
- Pages: 80-84
- Section: SHORT COMMUNICATIONS
- URL: https://microbiol.crie.ru/jour/article/view/35
- DOI: https://doi.org/10.36233/0372-9311-2016-2-80-84
- ID: 35
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Abstract
Aim. Evaluation of dysbiotic condition of intestine based on determination of local antioxidant index (LAI). Materials and methods. 155 patients with bacteriologically confirmed intestine dysbacteriosis were examined. 20 individuals with normobiocenosis of the intestine served as control. Feces dilution 10-2 was used for biochemical study. Catalase, superoxide dismutase activity and level of malonic dialdehyde was evaluated photometrically by the value of optical density of the samples. Microbial landscape of feces was studied by a bacteriologic method with parallel determination in samples of coprofiltrates of activity of the indicated enzymes and content of malonic dialdehyde. Evaluation of disturbances of microecology of the intestine was carried out by a biochemical method by calculating local antioxidant index in juxtaposition with microbial map data. Results. LAI parameter was significantly higher than 20 in coprofiltrates of people with intestine normobiocenosis. In patients with detected intestine dysbacteriosis, depending on its degree, LAI parameter changes were registered in the range from negative to 20. Dysbacteriosis in a part of patients had a prolonged character, LAI was lower than 14, that reflected the presence of a persistent inflammation. A tendency of normalization of microbial ecology and growth of LAI was observed during administration ofa probiotic preparation. Conclusion. A screening criteria is proposed, that differentiates the degree of severity of intestine dysbacteriosis based on calculation of local antioxidant index.
About the authors
M. N. Gapon
Rostov Research Institute of Microbiology and Parasitology
Author for correspondence.
Email: noemail@neicon.ru
Russian Federation
L. N. Ternovskaya
Rostov Research Institute of Microbiology and Parasitology
Email: noemail@neicon.ru
Russian Federation
References
- Бондаренко В.М. Роль условно-патогенных бактерий при хронических воспалительных процессах различной локализации. Тверь, Триада, 2011.
- Бондаренко В.М., Мацулевич Т.В. Дисбактериоз кишечника как клинико-лабораторный синдром: современное состояние проблемы. М., ГЭОТАР-медиа, 2007.
- Гапон М.Н., Терновская Л.Н. Показатели местной неспецифической резистентности при дисбактериозе толстой кишки. Журн. микробиол. 2010, 5: 53-57.
- Журавлева ТД. и др. Возрастные особенности свободно-радикального окисления липидов и антиоксидантной защиты в эритроцитах здоровых людей. Клин. лаб. дагн. 2002, 8: 17.
- Королюк М.А., Иванова Л.И. Метод определения активности каталазы. Лаб. дело. 1988, 1: 16-19.
- Микробиологическая диагностика дисбактериоза кишечника. Методические рекомендации. М., 2007.
- Отраслевой стандарт «Протокол ведения больных. Дисбактериоз кишечника». ОСТ 91500.11.0004-2003. Приказ МЗ РФ № 231 от 09.06.03.
- Сирота Т.В. Новый подход в исследовании процесса аутоокисления адреналина и использования его для измерения активности супероксиддисмутазы. Вопр. мед. химии. 1999, 3: 14-15.
- Стальная И.Д., Гаришвили Т.Г. Метод определения малонового диальдегида с помощью тиобарбитуровой кислоты. Современные методы в биохимии. М., Медицина, 1977.
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