N-ACETYL-β-D-GLUCOSAMINIDASE OF VIBRIO CHOLERAE

Aim. Study N-acetyl-β-D-glucosaminidase (chitobiase) (EC 3.2.1.30) in strains of Vibrio cholerae of O1/non-O1 serogroups of various origin, that is a component of chitinolytic complex taking into account object of isolation and epidemiologic significance of strains. Materials and methods. Cultures of V.cholerae O1/non-O1 serogroup strains were obtained from the museum of live culture of Rostov RIPC. Enzymatic activity analysis was carried out in Hitachi F-2500 fluorescent spectrophotometer using FL Solutions licensed software. NCBI databases were used during enzyme characteristics. Results. N-acetyl-β-D-glucosaminidase in V.cholerae O1/non-O1 serogroup strains was detected, purified by column chromatography, studied and characterized by a number of physical-chemical and biological properties. Comparative computer analysis of amino acid sequence of N-acetyl-β-D-glucosaminidases of V.cholerae (VC2217 gene), Serratia marcescens etc. has allowed to attribute the enzyme from V.cholerae to glycosyl-hydrolases (chi-tobiases) of family 20 and classify it according to enzyme nomenclature as EC 3.2.1.30. Conclusion. N-acetyl-β-D-glucosaminidase in V.cholerae of O1/non-O1 serogroups of various origin and epidemiologic significance, participating in chitin utilization was studied and characterized for the first time, and its possible role in biology of cholera causative agent was shown.

Vibrio cholerae, N-ацетил-β-D-глюкозаминидазная активность, 4-метилумбеллиферил-N-ацетил-β-D-глюкозаминид (4-MUF. GlcNAc), Vibrio cholerae, strains, N-acetyl-β-D-glucosaminidase activity, 4-methylumbelliferone-N-acetyl-β-D-glucosaminide (4-MUF. GlcNAc)