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Aim. Study N-acetyl-β-D-glucosaminidase (chitobiase) (EC in strains of Vibrio cholerae of O1/non-O1 serogroups of various origin, that is a component of chitinolytic complex taking into account object of isolation and epidemiologic significance of strains. Materials and methods. Cultures of V.cholerae O1/non-O1 serogroup strains were obtained from the museum of live culture of Rostov RIPC. Enzymatic activity analysis was carried out in Hitachi F-2500 fluorescent spectrophotometer using FL Solutions licensed software. NCBI databases were used during enzyme characteristics. Results. N-acetyl-β-D-glucosaminidase in V.cholerae O1/non-O1 serogroup strains was detected, purified by column chromatography, studied and characterized by a number of physical-chemical and biological properties. Comparative computer analysis of amino acid sequence of N-acetyl-β-D-glucosaminidases of V.cholerae (VC2217 gene), Serratia marcescens etc. has allowed to attribute the enzyme from V.cholerae to glycosyl-hydrolases (chi-tobiases) of family 20 and classify it according to enzyme nomenclature as EC Conclusion. N-acetyl-β-D-glucosaminidase in V.cholerae of O1/non-O1 serogroups of various origin and epidemiologic significance, participating in chitin utilization was studied and characterized for the first time, and its possible role in biology of cholera causative agent was shown.

About the authors

O. V. Duvanova

Rostov-on-Don Research Institute for Plague Control

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

B. N. Mishankin

Rostov-on-Don Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

A. S. Vodopianov

Rostov-on-Don Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

V. M. Sorokin

Rostov-on-Don Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation


  1. Aшмарин И.П. Статистические методы в микробиологических исследованиях. Л., Медицинская литература, 1962.
  2. Мишанькин Б.Н., Шиманюк Н.Я., Водопьянов С.О., Романова Л.В., Водопьянов А.С., Дуванова О.В., Атарова Г.Т., Демьяненко С.В. Изучение хитинолитического комплекса холерного вибриона сероварианта О139. Биотехнология. 2010, 1: 32-40.
  3. Bhowmick R., Ghosal A., Chatterjee N. S. Effect of environmental factors on expression and activity of chitinase genes ofvibrios with special reference to Vibrio choleraе. J. Appl. Microbiol. 2007, 103: 97-108.
  4. Henrissat B., Bairoch A. New familities in the classification of glycosyl hydrolase based on amino acid sequence similarities. Biochem. J. 1993, 293:781-788.
  5. Hunt D.E., Gevers D., Vahora N.M., Polz M.F. Conservation of the chitin utilization pathway in the Vibrionaceae. Appl. Environ. Microbiol. 2008, 74 (1): 44-51.
  6. Kaplan J.B., Ragunath C., Ramasubbu N., Fine D.H. Detachmеnt of Actinobacillus actinomycetemcomitans biofilm cells by an endogenous P-hexosaminidase activity. J. Bacteriol. 2003, 185 (16): 4693-4698.
  7. O’Brien M., Colwell R. A rapid test for chitinase activity that uses 4-methylumbelliferryl-N-acetyl-β-D-glucosaminide. Appl. Environ. Microbiol. 1987, 53 (7): 1718-1720.
  8. Tews I., Vincentelli R., Vorgias C. N-acetylglucosaminidsae (chitobiase) from Serrata marcescens: gene sequence, and protein production and purification in Escherichia coli. Gene. 1996, 170: 63-67.

Copyright (c) 2016 Duvanova O.V., Mishankin B.N., Vodopianov A.S., Sorokin V.M.

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