CERVICAL MUCUS NEUTROPHILS FUNCTIONAL STATUS INTERACTION WITH THE SPECIES COMPOSITION OF VAGINAL LACTOBACILLI IN PREGNANT WOMEN

Yu. S. Shishkova; Шишкова Ю. С.; V. F. Dolgushina; Долгушина В. Ф.; E. D. Grafova; Графова Е. Д.; S. A. Zavyalova; Завьялова С. А.; I. V. Kurnosenko; Курносенко И. В.; N. P. Evstigneeva; Евстигнеева Н. П.; K. G. Gromakova; Громакова К. Г.; O. L. Kolesnikov; Колесников О. Л.; A. V. Chukichev; Чукичев А. В.; I. I. Dolgushin; Долгушин И. И.

doi:10.36233/0372-9311-2018-4-51-56

CERVICAL MUCUS NEUTROPHILS FUNCTIONAL STATUS INTERACTION WITH THE SPECIES COMPOSITION OF VAGINAL LACTOBACILLI IN PREGNANT WOMEN

Authors: Shishkova Y.S.¹, Dolgushina V.F.¹, Grafova E.D.¹, Zavyalova S.A.², Kurnosenko I.V.¹, Evstigneeva N.P.³, Gromakova K.G.¹, Kolesnikov O.L.¹, Chukichev A.V.¹, Dolgushin I.I.¹
Affiliations:
1. South Ural State Medical University
2. State Clinical Outpatient Clinic No. 5
3. Ural Research Institute of Dermatology, Venerology and Immunopathology
Issue: Vol 95, No 4 (2018)
Pages: 51-56
Section: ИНФЕКТОЛОГИЧЕСКИЕ МЕХАНИЗМЫ СИМБИОТИЧЕСКИХ ВЗАИМОДЕЙСТВИЙ
Submitted: 10.04.2019
Published: 28.08.2018
URL: https://microbiol.crie.ru/jour/article/view/286
DOI: https://doi.org/10.36233/0372-9311-2018-4-51-56
ID: 286

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Abstract

Aim. Of the study is to assess cervical mucus neutrophils functional status depend on dominant lactobacillus strain in I trimester of pregnancy women. Material and methods. We definded 40 lactobacillus strains obtained from the genital tract of the pregnant women. We used mass spectrometry analysis to identify vaginal lactobacilli and evaluated biofilm formation. Also, we studied cervical mucus neutrophils viability, lysosomal activity, spontaneous and induced NBT test (Nitroblue Tetrazolium test), ability to absorb the latex particles. Isolated strains were divided into 3 groups depending on presence or absence of vaginal or cervical channel infectious diseases. Results. We identified 5 lactobacilli types: L. jensenii, L. mspatus, L. аcidophilus, L. gasseri, L. delbrueckii among 40 definded strains. We determined different intensity of phagocytosis depending on dominant strain in normocenosis. With the L. crispatus prevalence demonstrated the least intensity of phagocytosis compared with L. acidophilus and L.jensenii. Lactobacilli able to intensively produce biomatrix in condition of genital infection. Thus blocking antigenic potential and result in absence of NBT-reducing power of neutrophilic granulocytes and reduction antimicrobial protection of pregnant women genital tract mucosa. Conclusion. The indicators of neutrophils functional status depend on dominant lactobacilli strain in I trimester of pregnancy women.

Keywords

I триместр беременности, lactobacilli, neutrophils functional status, biofilm formation, I trimester of pregnancy

References

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Verstraelen H., Verhelst R., Claeys G. et al. Longitudinal analysis of the vaginal microflora in pregnancy suggests that L. crispatus promotes the stability of the normalvaginal microflora and that L. gasseri and/or L. iners are more conducive to the occurrence of abnormal vaginal microflora. BMC Microbiology. 2009, 9: 116.

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CERVICAL MUCUS NEUTROPHILS FUNCTIONAL STATUS INTERACTION WITH THE SPECIES COMPOSITION OF VAGINAL LACTOBACILLI IN PREGNANT WOMEN

Full Text

Abstract

Keywords

About the authors

References

Supplementary files

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