LARGE INTESTINE NORMOBIOCENOSIS AND PROOXIDANT-ANTIOXIDANT BALANCE OF COLONOCYTES, BLOOD PLASMA IN EXPERIMENTAL DISBIOOSIS AND USAGE OF RIOFLORA IMMUNO NEO PROBIOTIC

Aim. Study the changes in the composition of animals large intestine microbiocenosis and molecular-biochemical parameters of blood plasma and colonocytes in experimental gentamicin dysbiosis, possibility of correction using probiotic. Materials and methods. Drug dysbiosis was simulated by administration of gentamicin intraperitoneally and with correction aim injected probiotic. Quantitative and qualitative study of mucous microflora of the mice large intestine was performed by bacteriological method. The state of lipid peroxidation system was judged about by content of acylhydroperoxide and malonic dialdehyde, antioxidant protection system - by catalase and superoxide dismutase. Results. As a result of the study, a change in the composition of the intestinal microflora, a decrease in the enzymatic activity of antioxidant defense system in blood plasma and colonocytes, an increase in the content of lipid peroxidation products in blood plasma and colonocytes were recorded. The corrective effect of RioFlora Immuno Neo probiotic regarding the restoration of the intestine normobiocenosis and the molecular-biochemical parameters of animal colonocytes was noted. Conclusion. The use of the probiotic led to the restoration of microbial equilibrium in the intestinal microbiocenosis, and also had a positive effect on the superoxide dismutase activity of colonocytes, stabilization of the malonic dialdehyde content in the colon tissue.

large intestine mucous microflora, dysbiosis, antioxidant system, RioFlora Immuno Neo