Email this article VAGINAL LACTOBACILLI REGULATE THE ACTIVITY OF MURAMIDASE VIA HYDROGEN PEROXIDE AND SURFACTANTS
- Authors: Sgibnev A.V.1, Kremleva E.A.2
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Affiliations:
- Institute of Cellular and Intracellular Symbiosis
- Institute of Cellular and Intracellular Symbiosis, Orenburg State Medical University
- Issue: Vol 95, No 4 (2018)
- Pages: 21-27
- Section: СИМБИОТИЧЕСКИЕ ВЗАИМОДЕЙСТВИЯ НОРМОФЛОРЫ И ХОЗЯИНА
- URL: https://microbiol.crie.ru/jour/article/view/282
- DOI: https://doi.org/10.36233/0372-9311-2018-4-21-27
- ID: 282
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Abstract
Aim. Evaluation of the effect of metabolites of vaginal lactobacilli on the enzymatic and bactericidal activity of muramidase. Materials and methods. We investigated how the enzymatic and bactericidal activity of muramidase changes after treatment with supernatants of lactobacilli containing surfactants, hydrogen peroxide, or a combination thereof. The enzymatic activity was measured by the rate of Micrococcus luteus lysis, bactericidal activity for test strains of Escherichia coli and Lactobacillus acidophilus by results of seeding on agar plates. Results. The hydrogen peroxide and surfactants reduced the enzymatic activity of lysozyme. Bactericidal activity of lysozyme against L. acidophilus and E. coli was decreased under the influence of surfactants. Hydrogen peroxide and its combinations with surfactants decreased bactericidal activity of lysozyme against L. acidophilus and increased for E. coli. Low concentrations of surfactants potentiated the effect of H2O2 on the antibacterial activity of lysozyme. Conclusion. It is concluded that the metabolites of the normal microflora is a implement for modification of host defense factors in order to create favorable conditions for its own existence, and prevent introduction of allochthonous species.
About the authors
A. V. Sgibnev
Institute of Cellular and Intracellular Symbiosis
Author for correspondence.
Email: noemail@neicon.ru
Russian Federation
E. A. Kremleva
Institute of Cellular and Intracellular Symbiosis, Orenburg State Medical University
Email: noemail@neicon.ru
Russian Federation
References
- Бухарин О.В. Персистенция патогенных бактерий. М., 1999.
- Гланц С. Медико-биологическая статистика. М., Практика, 1998.
- Aldunate M., Srbinovski D., Hearps A.C. et al. Antimicrobial and immune modulatory effects of lactic acid and short chain fatty acids produced by vaginal microbiota associated with eubiosis and bacterial vaginosis. Frontiers in Physiology. 2015, 6: A164.
- Chao C.C., Ma Y.S., Stadtman E.R. Modification of protein surface hydrophobicity and methionine oxidation by oxidative systems. Proc. Natl. Acad. Sci USA. 1997, 94: 2969-2974.
- Dover S.E., Aroutcheva A.A., Faro S., Chikindas M.L. Natural antimicrobials and their role in vaginal health: a short review. International J. Probiotics & Prebiotics. 2008, 3 (4). 219-230.
- Ivanov R.A., Soboleva O.A., Smirnov S.A. et al. Effect of surfactants of different types on the bacteriolytic activity of lysozyme. Rus. J. Bioorg. Chem. 2015, 41 (3): 260-265.
- Kaewsrichan J., Peeyananjarassri K., Kongprasertkit J. Selection and identification of anaerobic lactobacilli producing inhibitory compounds against vaginal pathogens. FEMS Immunol. Med. Microbiol. 2006, 48 (1): 75-83.
- Nakata K., Tsuchido T., Matsumura Y Antimicrobial cationic surfactant, cetyltrimethylammonium bromide, induces superoxide stress in Escherichia coli cells. J. Applied Microbiology. 2011, 110 (2): 568-579.
- Nash J.A., Ballard T.N., Wfeaver T.E., Akinbi H.T. The peptidoglycan-degrading property of lysozyme is not required for bactericidal activity in vivo. J. Immunol. 2006, 177 (1): 519-526.
- Sgibnev A.V., Kremleva E.A. Vaginal protection by H2O2-producing lactobacilli. Jundishapur J. Microbiology. 2015, 8 (10): A e22913.
- Simonian M. H., Smith J. A. Spectrophotometric and colorimetric determination of protein concentration. Curr. Protoc. Mol. Biol. 2006, 76. 10.1A:10.1.1-10.1A.9.
- Valore E.V., Park C.H., Igreti S.L., Ganz T. Antimicrobial components of vaginal fluid. Am. J. Obstet. Gynecol. 2002, 187 (3): 561-568.
- Willumsen P.A., Karlson U. Screening ofbacteria, isolated from PAH-contaminated soils, for production of biosurfactants and bioemulsifiers. Biodegradation. 1997, 7 (5): 415-423.
- Wilson M. Microbial Inhabitants of Humans: Their Ecology and Role in Health and Disease. Cambridge University Press. 2005.
- Yarbrough V.L., Winkle S., Herbst-Kralovetz M.M. Antimicrobial peptides in the female reproductive tract: A critical component of the mucosal immune barrier with physiological and clinical implications. Human Reproduction Update. 2015, 21 (3): 353-377.
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