Cover Page

Cite item


Aim. To compare the species composition of lactobacilli in women of reproductive age using real time PCR on urogenital samples before and after culturing microorganisms in Blood-Serum-Y^ast extract-Agar (BSYA). Materials and methods. Using real time PCR, we have examined the species composition of vaginal lactobacilli in 25 healthy women of reproductive age. Samples of urogenital swabs (endocervical and vaginal swabs) were studied. Species identification has been carried out twice: in the native clinical material and in the samples received after using microorganism culture technique in BSYA. Results. After culturing vaginal microorganisms in BSYA, L. iners predominance was found only in 2 cases (8%). At the same time, when using native clinical material this species predominance was discovered in 10 samples (40%). Conclusion. When culturing lactobacilli in BSYA, the growth of L. iners is stunted compared to other species of vaginal lactobacilli. The use of cultural testing turned out to be ineffective in case of predominance of L. iners, the species associated with an increased risk of vaginal dysbiosis.

About the authors

E. S. Voroshilina

Ural State Medical University, «Garmonia» Medical Centre

Author for correspondence.
Russian Federation

D. L. Zornikov

Ural State Medical University

Russian Federation

L. G. Boronina

Ural State Medical University

Russian Federation


  1. Боронина Л.Г., Саматова Е.В. Патент РФ № 2011128466/10, 08.07.2011. Питательная среда для выделения, культивирования и определения гемолитических свойств бактерий из клинического материала. Патент России № 2481394 С2. 2013. Бюл. №13.
  2. Зорников Д.Л., Тумбинская Л.В., Ворошилина Е.С. Взаимосвязь отдельных видов лактобацилл с суммарной долей лактофлоры в вагинальном микробиоценозе и группами условно-патогенных микроорганизмов, ассоциированными с дисбиозом влагалища. Вестник уральской медицинской академической науки. 2015, 4 (55): 99-105.
  3. Кира Е.Ф., Бактериальный вагиноз. Санкт-Петербург, 2001.
  4. Atashili J., Poole C., Ndumbe P.M. et al. Bacterial vaginosis and HIV acquisition: a metaanalysis of published studies. AIDS. 2008 Jul 31;22(12):1493-501.
  5. Balashov S.V., Mordechai E., Adelson M.E. et al. Multiplex quantitative polymerase chain reaction assay for the identification and quantitation of major vaginal lactobacilli. Diagn. Microbiol. Infect. Dis. 2014 Apr;78(4):321-327.
  6. Brotman R.M., Klebanoff M.A., Nansel T.R. et al. Bacterial vaginosis assessed by gram stain and diminished colonization resistance to incident gonococcal, chlamydial, and trichomonal genital infection. J. Infect. Dis. 2010, 202:1907-1915.
  7. Dominguez-Bello M.G., Costello E.K., Contreras M. et al. Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns. Proc. Natl. Acad. Sci. USA. 2010 Jun 29;107(26):П971-П975.
  8. Falsen E., Pascual C., Sjoden B. et al. Phenotypic and phylogenetic characterization of a novel Lactobacillus species from human sources: description of Lactobacillus iners sp. nov. Int. J. Syst. Bacteriol. 1999, 49:217-221.
  9. Haggerty C.L., Hillier S.L., Bass D.C. et al. PID Evaluation and Clinical Health study investigators. Bacterial vaginosis and anaerobic bacteria are associated with endometritis.Clin. Infect. Dis. 2004 Oct 1;39(7):990-995.
  10. Lauer E., Helming C., Kandler O. Heterogeneity of the species Lactobacillus acidophilus (Moro) Hansen and Mocquot as revealed by biochemical characteristics and DNA-DNA hybridization. Zentbl. Bakteriol. Microbiol. Hyg. Abt. 1980, 1:150-168.
  11. Martin H.L., Richardson B.A., Nyange P.M. et al. Vaginal lactobacilli, microbial flora, and risk of human immunodeficiency virus type 1 and sexually transmitted disease acquisition. J. Infect. Dis. 1999, 180(6):1863-1868.
  12. Ness R.B., Kip K.E., Hillier S.L. et al. A cluster analysis of bacterial vaginosis-associated microflora and pelvic inflammatory disease. Am. J. Epidemiol. 2005 Sep 15;162(6):585-590.
  13. Rathod S.D., Krupp K., Klausner J.D. et al. Bacterial vaginosis and risk for Trichomonas vaginalis infection: a longitudinal analysis. Sex. Transm. Dis. 2011 Sep;38(9):882-886.
  14. Tamrakar R., Yamada T., Furuta I., et al. Association between Lactobacillus species and bacterial vaginosis-related bacteria, and bacterial vaginosis scores in pregnant Japanese women. ВМС Infect. Dis. 2007 Nov 7;7:128.
  15. Verstraelen H., Verhelst R., Claeys G. et al. Longitudinal analysis of the vaginal microflora in pregnancy suggests that L. crispatus promotes the stability of the normal vaginal microflora and that L. gasseri and/or L. iners are more conducive to the occurrence of abnormal vaginal microflora. BMC Microbiol. 2009 Jun 2;9:116.

Copyright (c) 2018 Voroshilina E.S., Zornikov D.L., Boronina L.G.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС77-75442 от 01.04.2019 г.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies