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Aim. To study the effect of N-acetyl-L-cysteine on biofilm of V. cholerae of different sero-groups isolated from various sources and with different epidemiological significance (the presence/ absence of the ctx AB genes and tcpA). Materials and methods. Bacterial eiiltiire of Vibrio cholerae El Tor O1 and O139 serogroups were grown as biolms. We have estimated the influence of the drug N-acetyl-L-cysteine at a concentration of 0.5 - 4 mg/ml on the formation, of the formed biofilm and in planktonic form. Results. Discovered antibacterial activity of N-acetyl-L-cysteine. Noted that it was influenced as in the formation, of the already formed biofilm and the planktonic form, the representatives of the Vibrio cholerae El Tor O1 and O139 serogroups in concentrations of 2 - 4 mg/ml, showing an antibacterial effect regardless of the presence/absence of genes ctx and tcpA AB. Conclusion. Identified the antibacterial action of the drug N-acetyl-L-cysteine against biofilm of V. cholerae indicates the desirability of considering the possibility of using drug therapy in cases variety of diseases caused by causative agents II - IV groups pathogenicity.

About the authors

O. V. Duvanova

Rostov-on-Don Research Institute for Plague Control

Author for correspondence.
Russian Federation

B. N. Mishankin

Rostov-on-Don Research Institute for Plague Control

Russian Federation

S. V. Titova

Rostov-on-Don Research Institute for Plague Control

Russian Federation

L. A. Korneeva

Rostov-on-Don Research Institute for Plague Control

Russian Federation


  1. Голуб А.В. Бактериальные биопленки - новая цель терапии? Клин. микробиол. и антимикроб. химиотер. 2012, 1: 23-29.
  2. Селянская Н.А., Титова С.В., Головин С.Н. и др. Действие антибактериальных препаратов на биопленки холерных вибрионов Эль Тор. Журн. микробиол. 2017, 2: 8-15.
  3. Титова С.В., Кушнарева Е.В. Оценка способности холерных вибрионов к образованию биопленок in vitro с помощью нового методического подхода. Фундаментальные исследования. 2014, 10: 375-379.
  4. Ушкалова Е. А. Ацетилцистеин в клинической практике: настоящее и перспективы. Фармацевтика. 2007, 17: 30-36.
  5. Aslam S., Darouiche R.O. Role of antibiofilm - antimicrobial agents in control of device - related infections. Int. J. Artif. Organs. 2011, 34 (9): 752-758.
  6. Huynh H.Q., Couper R.T., Tran C.D. et al. N-acetylcysteine, a novel treatment for Helicobacter pylory infection. Dig. Dis. Sci. 2004, 49: 1853-1861.
  7. Parry M.F., Neu H.C. Effect of N-acetylcysteine on antibiotic activity and bacterial growth in vitro. J. Clin. Microbiol. 1977, 5 (1): 58 -61.
  8. Prescott L.F., Illingworth R.N., Critchley J.A. et al. Intravenous N-acetylcystein : the treatment of choice for paracetamol poisoning. Brithish Med. J. 1979, 2: 1097-1100.
  9. Tamayo R., Patimalla B., Camilli A. Growth in a biophilm induces a hyperinfectious phenotype in Vibrio cholerae. Infect. Immun. 2010, 78 (8): 3560-3569.
  10. Zhao T., Liu Y. N-acetylcystein inhibit biofilms produced by Pseudomonas aeruginosa. J. Bacteriol. 2010, 10: 140.

Copyright (c) 2018 Duvanova O.V., Mishankin B.N., Titova S.V., Korneeva L.A.

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