Email this article THE CYTOKINE PROFILE OF MICE DENDRITIC CELLS UNDER THE INFLUENCE OF OprF AND aTox PROTEINS OF PSEUDOMONAS AERUGINOSA
- Authors: Akhmatova N.K.1, Kalinichenko E.O.1, Makarenkova I.D.2, Akhmatova E.A.1, Tukhvatulin A.I.3, Logunov D.Y.3, Mikhailova N.A.1
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Affiliations:
- Mechnikov Research Institute of Vaccines and Sera
- Somov Research Institute of Epidemiology and Microbiology
- Gamaleya National Research Centre of Epidemiology and Microbiology
- Issue: Vol 95, No 2 (2018)
- Pages: 15-22
- Section: ORIGINAL RESEARCHES
- Submitted: 10.04.2019
- Published: 28.04.2018
- URL: https://microbiol.crie.ru/jour/article/view/261
- DOI: https://doi.org/10.36233/0372-9311-2018-2-15-22
- ID: 261
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Abstract
Aim. To study the effect of OprF and aTox proteins of Pseudomonas aeruginosa on the cytokine profile of mice dendritic cells. Materials and methods. Dendritic cells (DC) were obtained from bone marrow cells of BALB/c mice when cultured with 20 ng/ml of recombinant GM-CSF and IL-4 (Biosource, USA). OprF and aTox of P. aeruginosa were used as the inducer of maturation of DC. The level of cytokines was determined in supernatants of DC using the Bio-Plex Pro™ Mouse Cytokine 23-plex Assay (BioRad, USA). Results. Evaluation of the profile and level of cytokines produced by dendritic cells of mice demonstrates the high activity of mature DC. Under the influence of recombinant proteins OprF+aTox, both large amounts of Th-1 cytokines were synthesized: IL-1a, IL-1P, IL-6, TNF-a, Th-2 cytokines: IL- 4, IL-10, IL-13, regulatory cytokines: IL-12, IFN-y, IL-17A and chemokines: KC (CXCL1), MIP-1a (CCL3), MIP-1e (CCL4), RANTES (CCL5). In our studies, we demonstrated the possibility of obtaining mature dendritic cells from the bone marrow of mice under the influence of a complex of P. aeruginosa antigens. Conclusion. The candidate Pseudomonas aeruginosa vaccine based on its recombinant proteins OprF and aTox induces the production of chemokines and Th-1, Th-2, Th-17 cytokines by mice dendritic cells.
About the authors
N. K. Akhmatova
Mechnikov Research Institute of Vaccines and Sera
Author for correspondence.
Email: noemail@neicon.ru
Россия
E. O. Kalinichenko
Mechnikov Research Institute of Vaccines and Sera
Email: noemail@neicon.ru
Россия
I. D. Makarenkova
Somov Research Institute of Epidemiology and Microbiology
Email: noemail@neicon.ru
Россия
E. A. Akhmatova
Mechnikov Research Institute of Vaccines and Sera
Email: noemail@neicon.ru
Россия
A. I. Tukhvatulin
Gamaleya National Research Centre of Epidemiology and Microbiology
Email: noemail@neicon.ru
Россия
D. Yu. Logunov
Gamaleya National Research Centre of Epidemiology and Microbiology
Email: noemail@neicon.ru
Россия
N. A. Mikhailova
Mechnikov Research Institute of Vaccines and Sera
Email: noemail@neicon.ru
Россия
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