Email this article CATHETER-ASSOCIATED URINARY TRACT INFECTION IN CHILDREN WITH CONGENITAL HEART AND KIDNEY DISEASE
- Authors: Orlova O.G.1, Rybalchenko O.V.1, Erman M.V.1, Pervunina T.M.2, Fonturenko A.Y.1
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Affiliations:
- St. Petersburg State University
- St. Petersburg State University, National Medical Research Center, St. Petersburg
- Issue: Vol 95, No 1 (2018)
- Pages: 23-31
- Section: ORIGINAL RESEARCHES
- URL: https://microbiol.crie.ru/jour/article/view/243
- DOI: https://doi.org/10.36233/0372-9311-2018-1-23-31
- ID: 243
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Abstract
Aim. To detect pathogens that are capable of causing diseases or infections as postoperative complications in children with congenital heart and kidney disease, to be effective at preventing catheter-associated urinary tract infection. Materials and methods. Clinical material served samples of excrement, urine and urinary catheters of Foley (Unomedica) from sixteen children with congenital heart disease, who were being treated at the Almazov Medical Research Center. The isolated strains were classified according to the identification RapID Systems (USA). Antibiotic sensitivity of isolated bacteria was performed by thedisk diffusion method (NICF, Russia). Scanning electron microscopy (SEM) imaging revealed several types of bacterial communities on intraluminal surface of urethral catheter. Escherichia coli strains were tested for presence of virulence genes a-hemolysin: hly А, В, C by real-time PCR testing (using the detecting amplifier DTlite (DNA Technology, Russia). Results. The main microbial agents were isolated in urines samples from 3 of 16 patients were Staphylococcus aureus and E. coli. Gram-positive cocci were found in 56% samples of excrement and Gram-negative in 44% samples. It was found that on intraluminal surface in 10 out of 16 urethral catheter samples mixed microbial cultures were isolated, S. aureus and E. coli were prevailed. Methicillin-resistant strains of S. aureus (MRSA) have been reported in 85% cases. E. coli a-hemolysin (Ыу А, В, C) were identified in 60% strains. SEM examination two samples of urinary catheters revealed actinomyces-like forms of bacteria that were not detected in the primary bacteriological study. Conclusion. Early diagnosis of catheter-associated urinary tract infections in children with congenital heart heart and kidney disease requires a comprehensive approach to setting detecting priorities and selecting the most effective microbiological analysis, and is mandatory to use modem diagnostic methods.
About the authors
O. G. Orlova
St. Petersburg State University
Author for correspondence.
Email: noemail@neicon.ru
Russian Federation
O. V. Rybalchenko
St. Petersburg State University
Email: noemail@neicon.ru
Russian Federation
M. V. Erman
St. Petersburg State University
Email: noemail@neicon.ru
Russian Federation
T. M. Pervunina
St. Petersburg State University, National Medical Research Center, St. Petersburg
Email: noemail@neicon.ru
Russian Federation
A. Yu. Fonturenko
St. Petersburg State University
Email: noemail@neicon.ru
Russian Federation
References
- Демикова Н.С., Лапина А.С. Врожденные пороки развития в регионах Российской Федерации (итоги мониторинга за 2000-2010 г.г.). Рос. вести, перинатол. педиатр. 2012, 2:91-98.
- Смертность населения Российской Федерации. 2012 г. (Статистические материалы Минздрава России). М., 2014.
- Эрман М.В., Балацкий С.Ю. Врожденные аномалии развития почек у детей. В кн.: Знание пропедевтики - основа клинического мышления педиатра. Под ред. В. В. Юрьева, В.П.Новиковой, А.С.Симаходского. Спб, ИнформМед, 2015, с. 148-164.
- Andersen L.B., Bank S., Hertz В. et al. Actinobaculum schaalii, a cause of urinary tract infections in children? Acta Paediatr. 2012, 101 (5): e232-234.
- Cocchi G., Magnani C., Morini M.S. et al. Urinary tract abnormalities (UTA) and associated malformations: data ofthe Emilia-Romagna Registry. IMER Group. Emilia-Romagna Registry on Congenital Malformations. Eur. J. Epidemiol. 1996, 12 (5): 493-497.
- Costerton J.W. Introduction to biofilm. Int. J. Antimicrob. Agents. 1999, 11 (3-4): 217-221. doi: 10.1016/S0924-8579(99)00018-7.
- Dolk H., Loane M., Game E. The prevalence of congenital anomalies in Europe. Adv. Experim. Med. Biol. 2010, 686: 349-364.
- Greenwood R.D., Rosenthal A., Nadas A.S. Cardiovascular malformations associated with congenital anomalies of the urinary tract system. Observations in a series of 453 infants and children with urinary system malformations. Clin. Pediat. 1976,15:1101-1104.
- Hoenigl M., Leitner E., Valentin T. et al. Endocarditis caused by Actinobaculum schaalii. Emerg. Infect. Dis. 2010,16:1171-1173.
- Kot B., Wicha J., Gruzewska A. et al. Virulence factors, biofilm-forming ability, and antimicrobial resistance of urinary Escherichia coli strains isolated from hospitalized patients. Turk. J. Med. Sci. 2016, 46 (6): 1908-1914. doi: 10.3906/sag-1508-105.
- Lotte L., Lotte R., Durand M. et al. Infections related to Actinotignum schaalii (formerly Actinobaculum schaalii): a 3-yearprospective observational study on 50 cases. Clin. Microbiol. Infect. 2015 Nov 6. http://dx.doi.Org/10.1016/j. cmi.2015.10.030. pii: S1198-743X (15)00955-6.
- Lawson P.A., Falsen E., Akervall E. et al. Characterization of some Actinomyces-like isolates from human clinical specimens: reclassification ofActinomyces suis (Soltys and Spratling) as Actinobaculum suis comb. nov. And description of Actinobaculum schaalii sp. nov. Int. J. Syst. Bacteriol. 1997,47: 899-903.
- Lotte R., Lotte L., Ruimy R. Actinotignum schaalii (formerly Actinobaculum schaalii): a newly recognized pathogen - review of the literature. Clin. Microbiol. Infect. 2016, 22: 2836.
- Pedersen H., Senneby E., Rasmussen M. Clinical and microbiological features of Actinotignum bacteremia:a retrospective observational study of 57 cases. Eur. J. Clin. Microbiol. Infect.Dis. 2017, 36: 791-796. doi: 10.1007/sl0096-016-2862-y.
- PajlrtD., Simoons-Smit A.M., Savelkoul P.H.M.etal. Pyelonephritis caused by Actinobaculum schaaalii in a child with pyeloureteraljunction obstruction. Eur. J. Clin. Microbiol. Infect. Dis. 2003, 22: 438-440.
- RosnerM.H., Portilla D., OkusaM. Cardiac surgery as a cause ofacute kidney injury: pathogenesis and potential therapies. J. Intens. Care Med. 2008, 23: 3-18.
- Tuuminen T., Suomala P., Haju I. Actinobaculum schaalii: identification with MALDI-TOF. New Microbes New Infect. 2014, 2: 38-41.
- Yassin A. F., SproerC., Pukall R. et al. Dissection ofthe genus Actinobaculum: Reclassification of Actinobaculum schaalii Lawson et al. 1997 and Actinobaculum urinale Hall et al. 2003 as Actinotignum schaalii gen. nov., comb. nov. and Actinotignum urinale comb, nov., description of Actinotignum sanguinis sp. nov. and emended descriptions of the genus Actinobaculum and Actinobaculum suis; and re-examination of the culture deposited as Actinobaculum massiliense CCUG 47753T (5DsM 19118T), revealing that it does not represent a strain of this species. Int. J. Syst. Evolution. Microbiol. 2015, 65:615-624 doi: 10.1099/ijs.0.069294-0.
- Zimmermann P., Berlinger L., Liniger B. et al. Actinobaculum schaalii an emerging pediatric pathogen? BMC Infect. Dis. 2012, 12:201.
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