FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE

R. B. Gorodnichev; Городничев Р. Б.; D. V. Rakitina; Ракитина Д. В.; A. I. Manolov; Манолов А. И.; Yu. P. Baykova; Байкова Ю. П.; P. L. Scherbakov; Щербаков П. Л.; G. B. Smirnov; Смирнов Г. Б.; E. N. Ilina; Ильина Е. Н.

doi:10.36233/0372-9311-2017-6-42-49

FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE

Authors: Gorodnichev R.B.¹, Rakitina D.V.¹, Manolov A.I.¹, Baykova Y.P.¹, Scherbakov P.L.¹, Smirnov G.B.¹, Ilina E.N.¹
Affiliations:
1. Federal Research and Clinical Center of Physical-Chemical Medicine
Issue: Vol 94, No 6 (2017)
Pages: 42-49
Section: Articles
URL: https://microbiol.crie.ru/jour/article/view/229
DOI: https://doi.org/10.36233/0372-9311-2017-6-42-49
ID: 229

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Abstract

Aim. To characterize pool of Crohn’s disease-associated E.coli isolated from patients with Crohn’s disease. Materials and methods. 28 clinical stains were selected. Clinical isolates, as well as control samples, were tested for their possible belonging to a certain phylogenetic group, the ability to adhere and invade on the model of a monolayer of CaCo2 epithelial cells, the ability to form biofilms and their mobility. Results. We have shown that E.coli, isolated from a different biomaterial, belonged to different phylogenetic groups and differed in their ability to form biofilms. Adhesive-invasive E.coli were found mainly in the material of biopsy of the intestinal mucosa of patients with Crohn’s disease. Conclusion. There are reasons to suppose that adhesive-invasive E.coli formed at the junction of ecological niches of the lumen and the intestinal wall.

Keywords

Crohn’s disease, adhesive-invasive E.coli, heterological models, biofilms, phylogenetic diversity of E.coli

References

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FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE

Full Text

Abstract

Keywords

About the authors

R. B. Gorodnichev

D. V. Rakitina

A. I. Manolov

Yu. P. Baykova

P. L. Scherbakov

G. B. Smirnov

E. N. Ilina

References

Supplementary files

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