IDENTIFICATION OF MYCOBACTERIA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY


Cite item

Full Text

Abstract

Aim. To compare results of mycobacteria identification by bacteriologic methods as well as by high-performance liquid chromatography (HPLC). Materials and methods. Two hundred and eighty strains of mycobacteria isolated from respiratory specimens and identified by bacteriologic methods and HPLC were studied. Results. It was established that results of HLPC use were highly correlated with results of microbiologic methods of mycobacteria identification: for identification of M.tuberculosis complex the correlation was 97.0%, for nontuberculous (NTM) slowly growing mycobacteria - 95.3%, for quickly growing NTM - 96.2% (overall - 96.1%). Results of identification of mycobacteria by HPLC were ready in significantly shorter time-frame (during 24 hours). Conclusion. HLPC method could be recommended for identification of mycobacteria in bacteriologic reference laboratories.

About the authors

M. V Makarova

Moscow Scientific and Practical Center for Tuberculosis Control, Russia

M. A Krasnova

Moscow Scientific and Practical Center for Tuberculosis Control, Russia

A. M Moroz

Moscow Scientific and Practical Center for Tuberculosis Control, Russia

References

  1. Оттен Т.Ф., Васильев А.В. Микобактериоз. СПб, Мед. пресса, 2005.
  2. Butler W., Jost K., Kilburn J. et al. Identification of mycobacteria by high-performance liquid chromatography. J. Clin. Microbiol. 1991, 29: 2468 - 2472.
  3. Butler W., Guthertz L. Mycolic acid analysis by high performance liquid chromatography for identification of Mycobacterium species. Clin. Microbiol. Rev. 2001, 14: 704 - 726.
  4. Castro C., Puerto G., Garcia L. et al. Molecular identification of non-tuberculous mycobacteria. Biomedica. 2007, 27: 439 - 446.
  5. Good R. New culture identification procedure initiated in CDC Mycobacteriology Lab. TB notes division of tb control, centers for disease control. Atlanta, 1990.
  6. Heifets L. Mycobacterial infections caused by nontuberculous mycobacteria. Sem. Res. Crit. Care Med. 2004, 25: 283 - 295.
  7. Kim H., Mun H. et al. Differentiation of Mycobacterial species by hsp65 duplex PCR followed by duplex-PCR-based restriction analysis and direct sequencing. J. Clin. Microbiol. 2006, 44: 3855 - 3862.
  8. Roth A., Reischl U., Streubel A. et al. Novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16S-23S rRNA gene spacer and restriction endonucleases. Ibid. 2000, 38: 1094 - 1104.
  9. Tortoli E., Bartoloni A., Burrini С. et al. Identification of nontuberculous mycobacteria: conventional tests versus HPLC of micolic acids: a four year experience. Clin. Mycobacteriol. Madrid, Prous-science, 1998.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2009 Makarova M.V., Krasnova M.A., Moroz A.M.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС77-75442 от 01.04.2019 г.