CHANGES OF EXPRESSION LEVELS OF INNATE IMMUNITY SIGNALING RECEPTORS DURING CANDIDA ALBICANS INFECTIONS IN VITRO AND IN VIVO


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Abstract

Aim. To study levels of expression of Toll-like receptors genes in response to Candida albicans antigens in vitro (using Vero cell line as well as mononuclear cells) and in vivo (using cervical canal cells of pregnant women). Materials and methods. Test-systems for measurement of expression levels of such genes as TLR1, TLR2, TLR6 as well as system for quantitative measurement of tumor necrosis factor α level, all of which were developed earlier, were used. Results. It was shown that antigens of C.albicans stimulated early increase of expression of innate immunity genes both in studied in vitro models and in cells of cervical canal of pregnant women with candidosis. Conclusion. Results of performed study allow to suggest that activation of innate immunity factors resulted from Candida albicans infection.

About the authors

O. A Gankovskaya

Mechnikov Research Institute of Vaccines and Sera; Russian State Medical University, Moscow, Russia

V. V Zverev

Mechnikov Research Institute of Vaccines and Sera; Russian State Medical University, Moscow, Russia

V. F Lavrov

Mechnikov Research Institute of Vaccines and Sera; Russian State Medical University, Moscow, Russia

L. P Blinkova

Mechnikov Research Institute of Vaccines and Sera; Russian State Medical University, Moscow, Russia

L. V Gankovskaya

Mechnikov Research Institute of Vaccines and Sera; Russian State Medical University, Moscow, Russia

P. A Kuznetsov

Mechnikov Research Institute of Vaccines and Sera; Russian State Medical University, Moscow, Russia

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Copyright (c) 2009 Gankovskaya O.A., Zverev V.V., Lavrov V.F., Blinkova L.P., Gankovskaya L.V., Kuznetsov P.A.

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