PREBIOTIC PROPERTIES OF MANNOSE AND ITS EFFECT ON SPECIFIC RESISTANCE

Aim. Study prebiotic properties of mannose and its effect on colonization resistance in experiments in
mice. Materials and methods. Experimental dysbiosis was induced by introduction into non-linear mice of
doxycycline hydrochloride. Prebiotic properties of mannose were studied by a single per oral administration
to mice of increasing doses of preparation for a week compared with probiotics lactobacterin and bifidumbacterin.
Lumen microflora was analyzed in feces. TNF-ƒ level was determined by using a commercial kit
ОртЕIА ELISA Kit. Phagocytic activity of neutrophils and macrophages was studied in a cytochemical test
of nitroblue tetrazolium reduction (NTT test) and by luminol-dependent chemiluminescence. Phagocytic
activity and digestive ability of alveolar macrophages was studied. Results. The ability of mannose along with
probiotic preparations bifidumbacterin and lactobacterin to restore the composition and numbers of indigenous
microflora of mice under the conditions of experimental dysbiosis was revealed. Per oral administration
of mannose and probiotic strains together with mannose was established to cause stimulating effect on functional
activity of macrophages increasing ingesting and digesting ability of the cells and facilitates reduction
of TNF-ƒ levels. Conclusion. Mannose has prebiotic effect; the ability of mannose to induce expression of
pro- and anti-inflammatory cytokines gives evidence of immunostimulating properties of the monosaccharide.

mannose, prebiotic properties, colonization resistance, dysbiosis, indigenous microflora