Email this article IMMUNOCORRECTION OF APOPTOGENIC EFFECT OF PERTUSSIS PREPARATIONS WITH NEUTROPHILOKINES
- Authors: Tyukavkina SY.1, Savis'ko AA1, Kharseeva GG1
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- Issue: Vol 89, No 5 (2012)
- Pages: 60-63
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/14199
- ID: 14199
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Abstract
Aim. Study the possibility of resistance increase of macrophages of experimental animals to apoptogenic
effect of pertussis preparations with neutrophilokines. Materials and methods. Apoptosis was evaluated in
Coulter flow cytofluorimeter after staining with propidium iodide, as well as by characteristic morphologic
changes in cells stained by histological preparations and by DNA fragmentation. Results. The studies performed
showed that pertussis preparations cause apoptosis of peritoneal macrophages leading to their alteration.
Neutrophilokines induced by pertussis preparations were also established to suppress macrophage apoptosis.
Conclusion. The results of our studies indicate the possibility to use neutrophilokines for immunocorrection
of macrophage apoptosis induced by pertussis preparations.
effect of pertussis preparations with neutrophilokines. Materials and methods. Apoptosis was evaluated in
Coulter flow cytofluorimeter after staining with propidium iodide, as well as by characteristic morphologic
changes in cells stained by histological preparations and by DNA fragmentation. Results. The studies performed
showed that pertussis preparations cause apoptosis of peritoneal macrophages leading to their alteration.
Neutrophilokines induced by pertussis preparations were also established to suppress macrophage apoptosis.
Conclusion. The results of our studies indicate the possibility to use neutrophilokines for immunocorrection
of macrophage apoptosis induced by pertussis preparations.
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References
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