Email this article STUDY OF POSSIBILITY OF FORMATION OF VIBRIO PARAHAEMOLYTICUS PANDEMIC CLONES BASED ON RETROSPECTIVE PCR-SCREENING OF CLINICAL STRAINS
- Authors: Monakhova EV1, Shalu OA1, Mazrukho AB1, Smolikova LM1, Nepomnyashchaya NB1
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Affiliations:
- Issue: Vol 89, No 5 (2012)
- Pages: 28-32
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/14177
- ID: 14177
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Abstract
Aim. PCR-genotyping of Vibrio parahaemolyticus strains that had caused sporadic diseases in Novorossiysk
from 1973 to 1976. Materials and methods. 24 clinical strains of V. parahaemolyticus isolated in Novorossiysk,
most of which belonged to serogroups O4:K12 and O4:K8; 10 O3:K6 strains - causative agents of gastroenteritis
outbreak in Vladivostok (1997) and 3 from Japan (1971) were used. PCR genotyping was performed
by a set of marker genes of 7 pathogenicity islands (VPaI-1 - VPaI-7) and a number of other pathogenicity
factors. Results. All the strains isolated in 1970s differed significantly by sets of VPaI marker genes. In contrast
to causative agents of outbreak in Vladivostok that contain all 7 VPaI genes (that is, members of the pandemic
group that had spread globally since 1996) none of the O4:K12 and O4:K8 Novorossiysk strains
contained the full set of all the VPaI genes. However this set was distributed among the members of the group.
Conclusion. Taking into account that O4:K12 and O4:K8 serogroups are considered by a number of authors
as O3:K6 serovariants, PCR-screening data obtained by us allows to assume that horizontal transfer of mobile
elements (VPaI) between strains circulating in the region could have led to the formation of pandemic clones
already in the 1970s. This implies that in several coastal regions in certain periods of time conditions that
favor these process may form, and risk of infection with pandemic clones is associated not only with import
of seafood.
from 1973 to 1976. Materials and methods. 24 clinical strains of V. parahaemolyticus isolated in Novorossiysk,
most of which belonged to serogroups O4:K12 and O4:K8; 10 O3:K6 strains - causative agents of gastroenteritis
outbreak in Vladivostok (1997) and 3 from Japan (1971) were used. PCR genotyping was performed
by a set of marker genes of 7 pathogenicity islands (VPaI-1 - VPaI-7) and a number of other pathogenicity
factors. Results. All the strains isolated in 1970s differed significantly by sets of VPaI marker genes. In contrast
to causative agents of outbreak in Vladivostok that contain all 7 VPaI genes (that is, members of the pandemic
group that had spread globally since 1996) none of the O4:K12 and O4:K8 Novorossiysk strains
contained the full set of all the VPaI genes. However this set was distributed among the members of the group.
Conclusion. Taking into account that O4:K12 and O4:K8 serogroups are considered by a number of authors
as O3:K6 serovariants, PCR-screening data obtained by us allows to assume that horizontal transfer of mobile
elements (VPaI) between strains circulating in the region could have led to the formation of pandemic clones
already in the 1970s. This implies that in several coastal regions in certain periods of time conditions that
favor these process may form, and risk of infection with pandemic clones is associated not only with import
of seafood.
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