Email this article PRESENCE OF GENES OF GENOTOXIN ASSOCIATED WITH pks PATHOGENICITY ISLAND IN ESCHERICHIA COLI M-17 PROBIOTIC STRAIN
- Authors: Bondarenko VM1, Fialkina SV1
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Affiliations:
- Issue: Vol 89, No 5 (2012)
- Pages: 25-27
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/14175
- ID: 14175
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Abstract
Aim. Study the presence of genetic determinants of pks pathogenicity island containing clb (colibactin)
genes in bacteria of the E. coli M-17 production strain by using PCR. Materials and methods. E.
coli M-17 cultures isolated from biopreparations bificol (Microgen) and colibacterin (Biomed) and
control strain obtained from Tarasevich State Institute on Standardization and Control (Moscow) were
studied. Detection of genetic markers of colibacterin was performed by using multiplex PCR with 4 pairs
of primers amplifying the main clb genes: clbB, clbN, clbA and clbQ, generating 575, 711, 981 and 820
bp amplicons, respectively. Results. In genome of all the studied E. coli M-17 strains сlbB, clbN, clbA
and clbQ genes associated with the formation of genotoxic colibacterin were detected. Conclusion.
Genome of E. coli M-17 bacteria used for production of probiotic preparations colibacterin and bificol
contains genetic determinants of genotoxin that require further studies in terms of evidence of harmlessness
of production bacteria.
genes in bacteria of the E. coli M-17 production strain by using PCR. Materials and methods. E.
coli M-17 cultures isolated from biopreparations bificol (Microgen) and colibacterin (Biomed) and
control strain obtained from Tarasevich State Institute on Standardization and Control (Moscow) were
studied. Detection of genetic markers of colibacterin was performed by using multiplex PCR with 4 pairs
of primers amplifying the main clb genes: clbB, clbN, clbA and clbQ, generating 575, 711, 981 and 820
bp amplicons, respectively. Results. In genome of all the studied E. coli M-17 strains сlbB, clbN, clbA
and clbQ genes associated with the formation of genotoxic colibacterin were detected. Conclusion.
Genome of E. coli M-17 bacteria used for production of probiotic preparations colibacterin and bificol
contains genetic determinants of genotoxin that require further studies in terms of evidence of harmlessness
of production bacteria.
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