INDEXES OF LOCAL CYTOKINE STATUS IN INDIVIDUALS WITH INTESTINE DYSBACTERIOSIS

Aim. Determination of the content of various cytokines in coprofiltrates of individuals with bacteriologically confirmed intestine dysbacteriosis. Materials and methods. Qualitative and quantitative content of large intestine microbiocenosis was studied; IFNy, pro-inflammatory (IL-2, IL-6, IL-8, IL-10) and anti-inflammatory (IL-4, IL-10) content was determined using EIA in coprofiltrates of 139 individuals aged 18 - 60 years. All the indexes were juxtaposed with the cytokine index (CI). Results. A high content of IL-2, IL-4 and IL-10 with normal levels of IL-10, IL-6 and IL-8 was established. A comparable content ofbifidobacteria, lactobacilli and escherichia was detected in individuals with various CI index; in individuals with CI above 1 c.u. and above 10 c.u., against the background of proportionally intensifying IFNy induction, an increase of quantity of escherichia with decreased enzymatic activity and frequent detection of opportunistic en-terobacteria, staphylococci and Candida genus fungi is noted. Conclusion. The presence of opportunistic microflora at low content of IFNy with CI of less than 1 c.u. could be evaluated as a dysbiotic reaction, and the presence of opportunistic microflora against the background of high IFNy content with CI of above 10 c.u. - as a development of systemic inflammation due to translocation of dysbiotic microflora into the bloodflow.

dysbacteriosis, gamma-interferon, cytokine index