GENETIC CHARACTERISTICS OF STAPHYLOCOCCUS AUREUS № 6 STRAIN - PRODUCER OF SECRETED PROTEIN-CONTAINING COMPOUNDS POSSESSING PROTECTIVE PROPERTIES

Aim. Genotype characteristics of Staphylococcus aureus № 6 strain that is a producer of a protective protein complex. Materials and methods. Features of structure of 9 genes, that code synthesis of pathogenicity factors, of S. aureus - spa, coa, seа, seв, se^ pvl, tst-h, mecA and scc-mecA, that are responsible for synthesis of protein A, coagulase, enterotoxins A, B and C, Panton-Valentine toxin (PVL), heat shock syndrome protein, resistance to methicillin and staphylococci chromosomal cassette, respectively, were studied by amplification in PCR of the respective gene fragments with subsequent conduction of direct sequencing. Results. The S. aureus № 6 strain under study possesses pvl gene fragments, as well as spa and coa genes, detected in all the studied strains, that belong to t12507 and ЕMRSA-16 types, respectively. Sea, seb, sec genes responsible for the synthesis of enterotoxins A, B and C were not detected in it, tst-h, mecA and scc-mecA gene fragments were not present. Conclusion. The detection of pvl gene fragment in the strain under study, on the one hand, and protective properties of the secreted protein-containing compound, on the other hand, give evidence in favor of the necessity of further analysis of extracellular proteome of S. aureus № 6.

Staphylococcus aureus, Staphylococcus aureus, polymerase chain reaction, sequencing, genes coding pathogenicity factors synthesis