FEATURES OF ADHESION OF ANAEROBIC PERIODONTOPATHOGENIC BACTERIA AND CANDIDA ALBICANS FUNGI TO EXPERIMENTAL SAMPLES OF BASIS DENTAL PLASTIC DEPENDING ON SURFACE ROUGHNESS AND POLISHING METHOD
- Authors: Tsarev V.N1, Ippolitov E.V1, Trefilov A.G1, Arutyunov S.D1, Pivovarov A.A1
-
Affiliations:
- Moscow State Medica1-Denta1 University, Russia
- Issue: Vol 91, No 6 (2014)
- Pages: 21-27
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.12.2014
- URL: https://microbiol.crie.ru/jour/article/view/13990
- ID: 13990
Cite item
Full Text
Abstract
Aim. Study the main surface parameters of mi11ed po1yacry1ic materia1s using atomic force microscopy and primary microbia1 adhesion of periodontopathogenic group bacteria and Candida albicans fungi taking into consideration the method of samp1e po1ishing. Materials and methods. Studied samp1es: mi11-treated without po1ishing (contro1); ergobox po1ished; po1ished in denta1 1aboratory conditions; po1ished by a rubber brush in dentists’ office. Microbia1 strains be1onging to periodontopathogenic species (c1inica1 iso1ates) that had been iso1ated from periodonta1 pockets of periodontitis patients: Porphyromonas gingivalis, Fusobacterium nucleatum, Streptococcus sanguis, C. albicans fungi were used for mode11ing experiments of primary adhesion of microbes to the materia1 samp1es. Results. S. sanguis had the highest degree of adhesion to po1ymer after mi11ing, P. gingivalis, C. albicans - medium, F. nucleatum - 1ow. A significant reduction of adhesion is observed during po1ishing in denta1 1aboratory conditions or ergobox, 1ess significant - during po1ishing in denta1 office. Conclusion. The data obtained a11ow to make a conc1usion that the samp1es from po1ymer materia1s for preparation of prosthesis basis have varying degree of intensity of microbia1 adhesion of members of periodontopathogenic microf1ora and C. albicans fungi that depends on the po1ishing method, that according1y determined the differences in co1o-nization resistance against formation of microbia1 biofi1m during po1ymer use in c1inica1 conditions.
About the authors
V. N Tsarev
Moscow State Medica1-Denta1 University, Russia
E. V Ippolitov
Moscow State Medica1-Denta1 University, Russia
A. G Trefilov
Moscow State Medica1-Denta1 University, Russia
S. D Arutyunov
Moscow State Medica1-Denta1 University, Russia
A. A Pivovarov
Moscow State Medica1-Denta1 University, Russia
References
- Арутюнов С.Д., Ибрагимов Т.И., Царев В.Н., Савкина Н.И., Трефилов А.Г. Микробиологическое обоснование выбора базисной пластмассы съемных зубных протезов. Стоматология. 2000, 3: 4-8.
- Кучерова М.А., Трефилов А.Г. Индекс адгезии микроорганизмов к полимерным базисным материалам как индикатор оценки антимикробных средств. Стоматолог. 2008, 5: 38-44.
- Мальков О.В., Литвиненко А.В. Измерение параметров шероховатости поверхности детали. М., Изд. МГТУ им. Баумана, 2012.
- Миронов В.Л. Основы сканирующей зондовой микроскопии. Н. Новгород, 2004.
- Царев В.Н., Давыдова М.М. Методы микробиологического исследования, применяемые в стоматологии. В: Микробиология, вирусология и иммунология. В.Н. Царев (ред.). М., Практическая медицина - ГЭОТАР-Медиа, 2009.
- Царев В.Н., Ибрагимов Т.И., Трефилов А.Г. Применение методов микробиологического мониторинга в процессе ортопедического лечения пациентов с вторичной полной адентией. Стоматолог. 2008, 2: 45-51.
- Binnig G., Quate C. F. Atomic force microscope. Physica1 Review Letters. 1986, 56 (9): 930933.
- Don1an R.M., Costertone J.W. Biofi1ms: surviva1 mechanisms c1inica11y re1evant microorganisms. C1in. Microbio1. Rev. 2002, 15 (2): 167-193.
- Eick S., G1ockmann E. Adherence of Streptococcus mutans to various restorative materia1s in a continuous f1ow system. J. Ora1. Rehabi1. 2004, 31: 278-285.
- Quirynen M., Bo11en C.M. The influence of surface roughness and surface-free energy on supra- and subgingiva1 p1aque formation in man. J. C1in. Periodonto1. 1995, 22: 1.