COLLECTION OF VIBRIO PARAHAEMOLYTICUS SPECIES MEMBERS: PHENOTYPIC AND GENOTYPIC CHARACTERISTICS


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Aim. Formation of Vibrio parahaemolyticus collection according to modern methodical opportunities and understanding of causative agent biology. Materials and methods. Traditional biochemical tests and PCR-testing of species-specific genes were used to confirm species membership. Catalase, DNAse, proteolytic and tweenase activity was determined by common methods. Virulence was evaluated by a complex method: hemolytic activity was determined in Kanagawa test (KT), urease - in Christensen medium, PRC-testing of tdh and trh genes. Serotyping was carried out with a commercial O/K-sera kit. PCR-genotyping was carried out by marker genes of 7 pathogenicity islands (VPaI-1-7). Results. Species membership was confirmed for the studied strains. Serologic typing allowed to detect members of 18 serologic groups among the collection strains. All the collection cultures were divided into 4 groups based on KT-Ure-tdh-trh features recombination. A number of genetic variants were detected, strains belonging to a pandemic group and O3:K6 serogroup were determined. Conclusion. A collection of V. parahaemolyticus cultures was formed and characterized by a large set of pheno- and genotypic features. A database was developed including information on strain origins, pheno- and genetic features, with genetic variants given, for ease of use of the collection.

About the authors

O. A Rykovskaya

Rostov Research Institute of Plague Control, Rostov-on-Don, Russia

L. M Smolikova

Rostov Research Institute of Plague Control, Rostov-on-Don, Russia

E. V Monakhova

Rostov Research Institute of Plague Control, Rostov-on-Don, Russia

O. S Chemisova

Rostov Research Institute of Plague Control, Rostov-on-Don, Russia

O. A Podoinitsina

Rostov Research Institute of Plague Control, Rostov-on-Don, Russia

E. N Golenischeva

Rostov Research Institute of Plague Control, Rostov-on-Don, Russia

E. M Sanamyants

Rostov Research Institute of Plague Control, Rostov-on-Don, Russia

M. M Sagakyants

Rostov Research Institute of Plague Control, Rostov-on-Don, Russia

R. R Dalikova

Rostov Research Institute of Plague Control, Rostov-on-Don, Russia

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Copyright (c) 2014 Rykovskaya O.A., Smolikova L.M., Monakhova E.V., Chemisova O.S., Podoinitsina O.A., Golenischeva E.N., Sanamyants E.M., Sagakyants M.M., Dalikova R.R.

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