MECHANISMS OF FORMATION OF POST-VACCINAL IMMUNE RESPONSE IN CHILDREN IMMUNIZED WITH APDT AND ADT-M PREPARATIONS

Aim. Study the mechanisms of formation of cell and humoral immunity against pertussis, diphtheria and tetanus in children immunized with immunobiological preparations (APDT vaccine and ADT anatoxin). Materials and methods. 30 practically healthy children (6 - 9 years of age) immunized with APDT and ADT-M preparations had TLR2, TLR4 expression determined in mononuclear cells (MNC). Vaccine preparations (APDT, ADT-M, AD-M, AT) and Corynebacterium diphtheriae gravis tox+, С. diphtheriae mitis tox- and Вordetellapertussis 345 were used as ligands. Cytokine production was determined in EIA. Content of anti-diphtheria, antitetanus and anti-pertussis antibodies - by PHA reaction and EIA. Results. During stimulation with vaccines and В. pertussis 345 strain MNC were characterized by an increase (p<0.05) of expression level of TLR2 and TLR4 and did not respond to stimulation with С. diphtheriae gravis tox+ and С. diphtheriae mitis tox- strains. Similar results were obtained during study of cytokine production (TNFa, IL-1, IL-6). A direct correlation between levels of antitoxic antibodies against diphtheria and tetanus (R=0.486), antibacterial antibodies against pertussis and diphtheria was detected (R=0.529). Conclusion. Analysis of cytokine production profile and determination of surface TLR expression can be used during evaluation of functional status of innate immunity cells and intensity of post-vaccinal immunity.

children, vaccination, anti-diphtheria, anti-pertussis, anti-tetanus immunity, cytokines, Toll-like receptors