Email this article MEASUREMENT OF GENITAL MYCOPLASMA CONCENTRATION BY USING A MICROBIOLOGICAL AND MOLECULAR-BIOLOGICAL METHODS
- Authors: Ivanova T.A1, Guschin A.E1, Gamova N.A2, Barkhatova O.I2, Rakovskaya I.V2
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Affiliations:
- Central Research Institute of Epidemiology, Moscow, Russia
- Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
- Issue: Vol 91, No 2 (2014)
- Pages: 25-29
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13926
- ID: 13926
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Abstract
Aim. Establishment of ratios that would allow to execute recalculation of mycoplasma concentration from CFU/ml and/or CCU/ml into units obtained during PCR analysis - geq/ml. Materials and methods. Pure cultures of Mycoplasma hominis, Ureaplasma parvum and Ureaplasma urealyticum were studied by cultural and molecular-biological methods with quantitative evaluation. Studies of initial cultures as well as series of 10-fold dilutions were carried out. 32 experiments in total were carried out. Results. Ratio between geq/ml and CFU/ml for M. hominis was 3.5; geq/ ml and CCU/ml ratio - 4.4. Ratio between geq/ml and CCU/ml for U. parvum was 7.1; for U. urealyticum - 11.2. Conclusion. Ratios between indexes obtained during quantitative study of pure genital micoplasma cultures by using 2 methods were established.
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About the authors
T. A Ivanova
Central Research Institute of Epidemiology, Moscow, Russia
A. E Guschin
Central Research Institute of Epidemiology, Moscow, Russia
N. A Gamova
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
O. I Barkhatova
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
I. V Rakovskaya
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
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