EFFECT OF LOW-INTENSITY RED LASER ON GROWTH OF STAPHYLOCOCCUS AUREUS AND SENSITIZING EFFECT OF PHOTODITAZIN

Cover Page


Cite item

Full Text

Abstract

Aim. Study the effect of laser emission in the red spectrum on growth of methicillin-sensitive and methicillin-resistant strains of Staphylococcus aureus, as well as photodynamic effect of photosensitizer photoditazin. Materials and methods. Effect of light of semiconductor red laser (X 660 nm, 100 mW/cm2) at 30, 60, 90 and 180 J/cm2 on growth of S. aureus colonies was determined. Time of exposure - 5, 10, 15 and 30 minutes. In certain series of experiments bacterial cells were sensitized in advance by a water solution of photoditazin at a concentration of 5x 1 O'6 M. Results. Red laser emission was established to cause a pronouncec suppression of bacterial growth. This effect on standard S. aureus strain only took place during use of relatively high exposure doses (180 J/cm2). Photosensitivity of methicillin-resistan: strain turned out to be significantly higher: bacteriostatic effect of red light was noted alreadx at the dose of 60 J/cm2. Treatment of bacterial cells with photoditazin in advance significantly enhanced growth-inhibiting effect of laser light.

About the authors

G. E. Bril

Razumovsky Saratov State Medical University

Author for correspondence.
Email: noemail@neicon.ru
Россия

A. V. Egorova

Razumovsky Saratov State Medical University

Email: noemail@neicon.ru
Россия

I. O. Bugaeva

Razumovsky Saratov State Medical University

Email: noemail@neicon.ru
Россия

G. V. Ponomarev

Orekhovich Institute of Biomedical Chemistry

Email: noemail@neicon.ru
Россия

References

  1. Braga E.D., Aguiar-Alves Е, de Freitas M.F. et al. High prevalence of Staphylococcus aureus and methicillin-resistant S. aureus colonization among healthy children attending public daycare centers in informal settlements in a large urban center in Brazil. BMC Infect. Dis. 2014, 6 (14): 538.
  2. Carrel М., Schweizer M.L., Sarrazin M.V. et al. Residential proximity to large numbers of swine in feeding operations is associated with increased risk of methicillin-resistant Staphylococcus aureus colonization at time of hospital admission in rural Iowa veterans. Infect. Control. Hosp. Epidemiol. 2014, 35 (2): 190-193.
  3. Dissemond J., Goos М., Esser S. Pathogenetic significance of methicillin resistant Staphylococcus aureus (MRSA) in chronic wounds. Vasa. 2003, 32 (3): 131-138.
  4. McKinnell J.A., Miller L.G., Eells S.J. et al. A systematic literature review and metaanalysis of factors associated with methicillin-resistant Staphylococcus aureus colonization at time of hospital or intensive care unit admission. Infect. Control. Hosp. Epidemiol. 2013,34(10): 1077-1086.
  5. Miller R.M., Price J.R., Batty E.M. et al. Healthcare-associated outbreak of meticillin-resistant Staphylococcus aureus bacteraemia: role of a cryptic variant of an epidemic clone. J. Hosp. Infect. 2014, 86 (2): 83-89.

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright (c) 2017 Bril G.E., Egorova A.V., Bugaeva I.O., Ponomarev G.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС77-75442 от 01.04.2019 г.