Email this article EFFECT OF LOW-INTENSITY RED LASER ON GROWTH OF STAPHYLOCOCCUS AUREUS AND SENSITIZING EFFECT OF PHOTODITAZIN
- Authors: Bril G.E.1, Egorova A.V.1, Bugaeva I.O.1, Ponomarev G.V.2
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Affiliations:
- Razumovsky Saratov State Medical University
- Orekhovich Institute of Biomedical Chemistry
- Issue: Vol 94, No 2 (2017)
- Pages: 34-37
- Section: ORIGINAL RESEARCHES
- URL: https://microbiol.crie.ru/jour/article/view/139
- DOI: https://doi.org/10.36233/0372-9311-2017-2-34-37
- ID: 139
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Abstract
Aim. Study the effect of laser emission in the red spectrum on growth of methicillin-sensitive and methicillin-resistant strains of Staphylococcus aureus, as well as photodynamic effect of photosensitizer photoditazin. Materials and methods. Effect of light of semiconductor red laser (X 660 nm, 100 mW/cm2) at 30, 60, 90 and 180 J/cm2 on growth of S. aureus colonies was determined. Time of exposure - 5, 10, 15 and 30 minutes. In certain series of experiments bacterial cells were sensitized in advance by a water solution of photoditazin at a concentration of 5x 1 O'6 M. Results. Red laser emission was established to cause a pronouncec suppression of bacterial growth. This effect on standard S. aureus strain only took place during use of relatively high exposure doses (180 J/cm2). Photosensitivity of methicillin-resistan: strain turned out to be significantly higher: bacteriostatic effect of red light was noted alreadx at the dose of 60 J/cm2. Treatment of bacterial cells with photoditazin in advance significantly enhanced growth-inhibiting effect of laser light.
About the authors
G. E. Bril
Razumovsky Saratov State Medical University
Author for correspondence.
Email: noemail@neicon.ru
Russian Federation
A. V. Egorova
Razumovsky Saratov State Medical University
Email: noemail@neicon.ru
Russian Federation
I. O. Bugaeva
Razumovsky Saratov State Medical University
Email: noemail@neicon.ru
Russian Federation
G. V. Ponomarev
Orekhovich Institute of Biomedical Chemistry
Email: noemail@neicon.ru
Russian Federation
References
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