Email this article EFFECT OF LOW-INTENSITY RED LASER ON GROWTH OF STAPHYLOCOCCUS AUREUS AND SENSITIZING EFFECT OF PHOTODITAZIN
- Authors: Bril G.E.1, Egorova A.V.1, Bugaeva I.O.1, Ponomarev G.V.2
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Affiliations:
- Razumovsky Saratov State Medical University
- Orekhovich Institute of Biomedical Chemistry
- Issue: Vol 94, No 2 (2017)
- Pages: 34-37
- Section: ORIGINAL RESEARCHES
- URL: https://microbiol.crie.ru/jour/article/view/139
- DOI: https://doi.org/10.36233/0372-9311-2017-2-34-37
- ID: 139
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Abstract
Aim. Study the effect of laser emission in the red spectrum on growth of methicillin-sensitive and methicillin-resistant strains of Staphylococcus aureus, as well as photodynamic effect of photosensitizer photoditazin. Materials and methods. Effect of light of semiconductor red laser (X 660 nm, 100 mW/cm2) at 30, 60, 90 and 180 J/cm2 on growth of S. aureus colonies was determined. Time of exposure - 5, 10, 15 and 30 minutes. In certain series of experiments bacterial cells were sensitized in advance by a water solution of photoditazin at a concentration of 5x 1 O'6 M. Results. Red laser emission was established to cause a pronouncec suppression of bacterial growth. This effect on standard S. aureus strain only took place during use of relatively high exposure doses (180 J/cm2). Photosensitivity of methicillin-resistan: strain turned out to be significantly higher: bacteriostatic effect of red light was noted alreadx at the dose of 60 J/cm2. Treatment of bacterial cells with photoditazin in advance significantly enhanced growth-inhibiting effect of laser light.
About the authors
G. E. Bril
Razumovsky Saratov State Medical University
Author for correspondence.
Russian Federation
A. V. Egorova
Razumovsky Saratov State Medical UniversityRussian Federation
I. O. Bugaeva
Razumovsky Saratov State Medical UniversityRussian Federation
G. V. Ponomarev
Orekhovich Institute of Biomedical ChemistryRussian Federation
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