CHARACTERISTICS OF ENTEROHEMORRHAGIC ESCHERICHIA COLI О145:Н28 ISOLATED FROM A PATIENT WITH HEMOLYTIC-UREMIC SYNDROME


Cite item

Full Text

Abstract

Aim. Determine etiologic significance of clinical strains of E. coli О145:Н28 isolated from feces of a patient with hemolytic-uremic syndrome (HUS). Materials and methods. 20 E. coli strains isolated from feces of a patient with HUS that developed after an acute intestine infection were studied. Antigenic structure of strains was determined by sequencing of rfb and fliC genes; presence of virulence genes (pap, aaf, sfa, afa, eaeА, bfpA, ial, hly, cnf, stx1, stx2, LT, ST and aer) - in PCR; ESBL production - by double disk method, ESBL genes - in PCR. Results. The strains contained rfb gene coding О145, fliC gene coding H7. Genes coding synthesis of stx2-toxin and intimin (eaeA) were detected. The strains were resistant to P-lactams due to production of CTX-M class ESBL. Conclusion. A causative agent E. coli О145:Н28 was isolated from a patient with HUS that produces stx2-toxin and CTX-M class ESBL and has not been previously registered in Russian Federation.

Keywords

About the authors

L. A Kaftyreva

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

M. A Makarova

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

T. A Konovalova

Central Research Institute of Epidemiology, Moscow, Russia

Z. N Matveeva

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

References

  1. Кафтырева Л.А., Егорова С.А., Макарова М.А. и др. Характеристика биологических свойств E.coli 0104:H4 - возбудителя крупной пищевой вспышки, возникшей в Германии в мае 2011 г. Клин. лаб. диагн. 2012, 1: 44-46.
  2. Afset J., Bevanger L., Romundstad P, Bergh K. Association of atypical enteropathogenic Esherichia coli (EPEC) with prolonged diarrhea. J. Med. Microbiol. 2004, 53: 1137-1144.
  3. Bielaszewska M., Karch N. Non-O157:H7 Shiga toxin verocyto-toxin-producing Escherihia coli strains: epidemiological significance and microbiological diagnosis. World J. Microbiol. Biotechnol. 2000, 16: 711-718.
  4. Blanco J.E., Blanco M., Alonso M.P. еt al. Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)-producing Escherichia coli isilates from human patients: prevalence in Lugo, Spain, from 1992-1999. J. Clin. Microbiol. 2004, 42: 311-319.
  5. Brooks J.T., Sowers E.G., Wells J.G. et al. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. J. Infect. Dis. 2005, 192: 1422-1429.
  6. De Schrijver K., Buvens G., Posse B. at al. Outbreak of verocytotoxin-producing E. coli O145 and O26 infections associated with the consumption of ice cream produced at a farm, Belgium, 2007. Eurosurveillance. 2008, 13:8041. Available at: http://eurosurveillance.org/ViewArticle aspx?Articleid=8041.
  7. Egorova S., Kaftyreva L., Grimont P.D.,Weill F-X. Prevalence and characterization of extended-spectrum cephalosporin-resistant nontyphoidal Salmonella isolates in adults in Saint Petersburg, Russia (2002-2005). Microbial Drug Resistance. 2007,13 (2): 102-107.
  8. Eklund M., Scheutz F, Siitonen A. Clinical isolates of non-O157 Shiga toxin-producing Escherihia coli: serotypes, virulence characteristics, and molecular profiles of strains of the same serotype. J. Clin. Microbiol. 2001, 39: 2829-2834.
  9. Johnson James R. Virulence factors in Escherihia coli. J. Clin. Microbiol. 2005, 43 (12): 62216222.
  10. Mead P.S., Slutsker L., Dietz V. еt al. Food-related illness and death in the United States. Emerg. Infect. Dis. 1999, 5: 607-625.
  11. Montenegro M.A., Bulte M., Trumpf T. et al. Detection and characterization of fecal verotoxin-producing Escherichia coli from healthy cattle. J. Clin. Microbiol. 1990, 28: 1417-1421.
  12. Padola N.L., Blanco M., Etheverria A.I. еt al. First isolation of enterohaemorrhagic Escherichia coli O145:H from cattle in feedlot in Argentina. BMC Microbiology. 2002, 2: 2-6.
  13. Piva I., Pereira A., Ferraz L. еt al. Virulance markers of enteroaggregative Escherichia coli isolated from children and adults with diarrhea in Brasilia. J. Clin. Microbiol. 2003, 41 (5): 18271832.
  14. Sonntag A.K., Prager R., Bielaszewska M. еt al. Phenotypic and genotypic analyses of enterohem-orrhagic Escherichia coli O145 strains from patients in Germany. J. Clin. Microbiol. 2004: 954962.
  15. Tozzi A.E., Caprioli A.F., Minelli F.A. еt al. Hemolytic uremic syndrome study group. Shiga toxin-producing Escherichia coli infections associated with hemolytic uremic syndrome, Italy, 1988-2000. Emerg. Infect. Dis. 2003, 9: 106-108.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2013 Kaftyreva L.A., Makarova M.A., Konovalova T.A., Matveeva Z.N.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС77-75442 от 01.04.2019 г.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies