IDENTIFICATION OF MICROORGANISMS BASED ON THE EFFECT OF GIANT RAMAN SCATTERING

Aim. Development and evaluation of express diagnostics of microorganisms for reduction of time spent on study execution. Materials and methods. Giant Raman scattering effect and proposed substrate with metallic nano-balls of silver were used for identification and indication of microorganisms. Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus saprophyticus, Candida albicans, Escherichia coli, Bacillus subtillis, Pseudomonas aeruginosa were used as objects of study. All the microorganisms were cultivated in dense nutrient media and non-fluorescent microbiological membrane filters. Results. Giant Raman scattering method on the substrates used increases sensitivity of indication and specificity of identification of microbes compared with other existing express methods and allows to see the signal from a single bacterium when exposed to laser light. The methodic proposed was also used to determine sensitivity of bacteria to antimicrobial preparations. Conclusion. The proposed hardware and method may be used for express diagnostics of processes of microbial nature.

giant Raman scattering, express diagnostics