EXPERIMENTAL INTERACTION OF HALOPHILIC PROKARYOTES AND OPPORTUNISTIC BACTERIA IN BRINE


Cite item

Full Text

Abstract

Aim. Study the effect of extremely halophilic archaea and moderately halophilic bacteria on preservation of opportunistic bacteria in brine. Materials and methods. 17 strains of moderately halophilic bacteria and 2 strains of extremely halophilic archaea were isolated from continental hypersaline lake Razval of Sol-Iletsk area of Orenburg Region. Identification of pure cultures of prokaryotes was carried out taking into account their phenotype properties and based on determination of16S RNA gene sequence. The effect ofhalophilic prokaryote on elimination of Escherichia coli from brine was evaluated during co-cultivation. Antagonistic activity of cell extracts of the studied microorganisms was evaluated by photometric method. Results. A more prolonged preservation of an E. coli strain in brine in the presence of live cells of extremely halophilic archaea Halorubrum tebenquichense and moderately halophilic bacteria Marinococcus halophilus was established. Extracts of cells of extremely halophilic archaea and moderately halophilic bacteria on the contrary displayed antagonistic activity. Conclusion. The protective effect of live cells of halophilic prokaryotes and antagonistic activity of their cell extracts change the period of conservation of opportunistic bacteria in brine that regulates inter-microbial interactions and changes the period of self-purification that reflects the sanitary condition of a hypersaline water body.

About the authors

E. A Selivanova

Research Institute of Cellular and Intracellular Symbiosis, Orenburg; Orenburg State Medical Academy, Russia

N. V Nemtseva

Research Institute of Cellular and Intracellular Symbiosis, Orenburg; Orenburg State Medical Academy, Russia

References

  1. Бухарин О.В., Валышев А.В., Елагина Н.Н. и др. Способ определения антилизоцимной активности микроорганизмов. Патент РФ № 2126051, 1999.
  2. Бухарин О.В., Плотников А.О., Немцева Н.В., Сгибнев А.В. Способ определения антигистоновой активности микроорганизмов. Патент РФ № 2065879, 1996.
  3. Бухарин О.В., Черкасов С.В., Сгибнев А.В. и др. Влияние микробных метаболитов на активность каталазы и рост Staphylococcus aureus 6538 P. Бюлл. экспер. биол. и мед. 2000, 130 (7): 80-82.
  4. Льюис К. Персистирующие клетки и загадка выживания биопленок. Биохимия. 2005, 70 (2): 327-336.
  5. Немцева Н.В., Селиванова Е.А., Плотников А.О. Роль симбиотических взаимодействий в выживании микроорганизмов в гипергалинных водоемах. Журн. микробиол. 2006, 4: 117-120.
  6. Немцева Н.В., Селиванова Е.А., Яценко-Степанова Т.Н., Игнатенко М.Е. Структура альго-планктона в Соль-Илецких озерах с различным уровнем солености. Известия ПГПУ им. В.Г. Белинского. 2011, 25: 531-537.
  7. Селиванова Е.А. Симбиотические связи микроорганизмов в планктонных сообществах соленых водоемов. Автореф. дис. канд. мед. наук. Оренбург, 2007.
  8. Селиванова Е.А. Структура и динамика бактериопланктона Соль-Илецких гипергалинных и мезогалинных озер. Вестник ОГУ. 2011, 4: 53-56.
  9. Селиванова Е.А., Немцева Н.В. Персистентные свойства микроорганизмов, обитающих в высокоминерализованных водоемах. Журн. микробиол. 2012, 4: 62-66.
  10. Gonzalez-Hernandez J.C, Jimenez-Estrada M., Peсa A. et al. Comparative analysis of trehalose production by Debaryomyces hansenii and Saccharomyces cerevisiae under saline stress. Extremophiles. 2005, 9: 7-16.
  11. Grosskopf R., Janssen PH., Liesack W Diversity and structure of the methanogenic community in anoxic rice paddy soil microcosms as examined by cultivation and direct 16S rRNA gene sequence retrieval. Appl. Environ. Microbiol. 1998, 64: 960-969.
  12. Jebbar M., Talibart R., Gloux K. et al. Osmoprotection of Escherichia coli by ectoin: uptake and accumulation characteristics. J. Bacteriol. 1992, 174: 5027-5035.
  13. Leuko S., Goh F., Allen M.A. et al. Analysis of intergenic spacer region length polymorphisms to investigate the halophilic archaeal diversity of stromatolites and microbial mats. Extremophiles. 2007, 11: 203-210.
  14. Oren A. The ecology of the extremely halophilic archaea. FEMS Microbiology Reviews. 1994, 13: 415-440.
  15. Vermeulen V., Kunte H.J. Marinococcus halophilus DSM 20408T encodes two transporters for compatible solutes belonging to the betaine-carnitine-choline transporter family: identification and characterization of ectoine transporter EctM and glycine betaine transporter BetM. Extremophiles. 2004, 8: 175-184.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2013 Selivanova E.A., Nemtseva N.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС77-75442 от 01.04.2019 г.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies